【摘要】 目的探讨胆囊收缩素受体CCKB-R mRNA在幼龄厌食大鼠结肠平滑肌细胞中的表达及加减益胃汤对其表达的影响。方法借助特制饲料喂养幼龄厌食大鼠模型，采用RT-PCR技术检测CCKB-R在幼龄厌食大鼠结肠平滑肌细胞中的表达，并观察加减益胃汤对该模型CCKB-R表达的影响。结果正常大鼠结肠平滑肌细胞中CCKB-R有低水平表达，特制饲料喂养造模后其表达水平异常增高，与空白对照组比较有极显著的统计学意义（P＜0.001）；加减益胃汤高、中、低剂量组均能显著抑制或下调CCKB-R的高表达，且有一定的量-效趋势。结论CCKB-R基因高表达可能是厌食发生的主要病理环节之一，抑制或下调CCKB-R基因表达可能是加减益胃汤治疗厌食症的主要作用靶点之一。
【关键词】 胆囊收缩素受体；厌食
Expression of CCKB-R mRNA in Colon Smooth Muscle Cells of the Anorexia Pedo-rats
ZHAO Qiong, LIAO Min, DING Weijun, LI Xiuliang, QU Jia
Chengdu University of TCM, Sichuan Chengdu 611137, China
Abstract：ObjectiveTo investigate the expression of CCKB-R mRNA in colon smooth muscle cells of the anorexia pedo-rats, and the effect of modified Yi Wei Decoction on it.MethodsThe anorexia rat model was establisbed by feeding rats with specially prepared forage. The expression of CCKB-R mRNA in colon smooth muscle cells of the anorexia pedo-rats was examined by RT-PCR, and the effect of modified Yiwei decoction on it was observed.ResultsCompared with blank control, the expression of CCKB-R mRNA in anorexia rats was significantly increased (P＜0.001). Highdosage, middledosage or lowdosage of Yiwei decoction all could obviously decrease the expression of CCKB-R mRNA as compared with model group(P＜0.001).ConclusionThe expression of CCKB-R mRNA can be obviously increased in colon smooth muscle cells of the anorexia pedo-rats, which is one of the mechanisms of anorexia.Modified Yiwei decoction can decrease the expression of CCKB-R mRNA in colon smooth muscle cells of the anorexia rats.
Key words：CCKB-R; Anorexia
近年来研究证实胆囊收缩素（CCK）等脑肠肽在小儿厌食症的发病中起着重要的作用，但对其受体表达及进一步的分子信号转导机制还不十分清楚。本研究在近年来中药复方加减益胃汤治疗小儿厌食症取得良好临床疗效和我们前期研究［1］的基础上，借助特制饲料喂养幼龄厌食大鼠模型，对CCKB-R在幼龄厌食大鼠结肠平滑肌细胞中的表达及加减益胃汤对其表达的影响进行了初步研究。现报道如下。
1 材料与仪器
加减益胃汤：北沙参15 g，麦冬10 g，乌梅5 g，山药15 g
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