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血管铸型观察bFGF对大鼠颌面部移植脂肪新生血管的影响 |
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廖天安 王琼超 谢富生 常杰
Observation on the function of basic fibroblast growth factor in free fat graft
revascularization of maxillofacial region by vascular cast
Liao Tiannan,Wang Qiongchao,Xie Fusheng,et al
【摘要】 目的 为探讨碱性成纤维细胞生长因子(bFGF)在自体游离脂肪中血管形成的
作用。方法 以SD大鼠双侧嚼肌区皮下作为受植床，取腹股沟区脂肪切碎后，实验侧加
入bFGF，并以纤维蛋白作为bFGF的缓释剂；对照侧加入单纯纤维蛋白进行移植。术后7天，
经心脏主动脉插管，以液体铸型材料甲烯丙烯酸甲脂、丙烯酸甲酯灌注制作移植体血管制作后，
在JSM-T300型扫描电镜下观察血管走向、形态并计量分析。结果 移植脂肪内血管多从颈
向头方行走，皮肤侧比嚼肌侧丰富，实验侧大量血管呈芽突状，芽突顶端及侧方有深而长的
椭圆或圆形内皮细胞核压迹，新生血管明显偏粗。结论 bFGF作为外源性因子加入自体游离
移植脂肪内可诱导血管长入，增加移植组织中血管的数量及增大新生血管直径的作用。
【关键词】 脂肪移植 碱性成细胞生长因子 血管铸型
Observation on the function of basic fibroblast growth factor in free fat graft revascularization
on maxillofacial regionby vascular cast Liao Tianan,Wang Qiongchao,Xie Fusheng,et al.Department
of Stomatology, Hai Nan Peoples Hospital, Hai Nan 570311
【Abstract】 Objective To search the function of basic fibroblast growth factor (bFGF) in vascularization
of auto-free grafting.Method Bilateral subcutaneous masseteric muscle region were used as the transplant
beds. The fat graft obtained from the inguinal region which were granulated and mixed with only fibroprotein
of the contrast side and fibroprotein containing with basic fibroblast growth factor (bFGF) of the experimental
side.The vascular casts of graft were prepared by methacrylate and methyl methacrylate monomar through
intubation to aorta via the heart 7 days postoperatively. The shape, quantity and direction of revascularization
were studied by JSM-T300 scanning electron microscopy.Result The direction of vesselsin graft went mainly
from neck to head. It was more abundance on subcutaneous tissue than adjacent muscular side. There were a
great number of vascular buds as predminant source of new vessels with spheriodal or spherical impressions of
nuclei of endethelial cells on [1] [2] 下一页
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