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白细胞介素1 肿瘤坏死因子 在膝关节原发性骨关节病发病中的作用 |
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何耀华 翁习生 邱贵兴
【摘要】目的探讨白细胞介素1β(IL－1β)、肿瘤坏死因子α（TNF－α）在膝关节原发性骨关节病(OA)发病中的作用。方法提取16例OA患者(OA组)、5例对照者(非OA组)的关节液，采用酶联免疫吸附法(ELISA法)测定IL－1β,生物活性法(MTT法)检测TNF－α的水平。结果在OA组关节滑液中，IL－1β的水平均显著高于对照组(P＜0.001);对照组的TMF-未能测出,而OA组TNF-α的检出阳性率为25.其次,为进一步探讨滑液中上述细胞因子的产生来源,传代培养膝关节滑膜细胞并用脂多糖(LPS)刺激,OA组(20例)关节滑膜细胞自发分泌IL-1β的水平显著高于对照组(5例),P＜0.05;LPS刺激的条件下,OA组和对照组中均显著升高,尤以OA组为著(P＜0.001);TNF-α在OA组中仅有两例呈阳性,对照组中均匀为阴性.结论 IL-1β和TNF-α在骨关节病的发病中起重要作用.OA患者滑膜细胞高水平自发性分泌IL-1β可能与其炎症发生与发展密切相关,可能是关节滑液中IL-1β的主要来源,而滑膜细胞分泌TNF-a可能不是关节滑液中的主要来源.
【关键词】骨关节炎 膝关节 白细胞介素1 肿瘤坏死因子
An Investigation of the Role Played by IL－1β and TNF－α on the Pathogenesis of Osteoarthrosis of the Knee
HE Yaohua,WENG Xisheng,QIU Guixing.
Department of Orthopaedics,Peking Union Medical College Hospital,Peking Union Medical College,Beijing 100730
【Abstract】 Objective To study the relationship between IL－1β , TNF－α and pathogenesis of knee osteoarthrosis. Methods IL－1β and TNF－α were measured concurrently in synovial fluid samples from 16 patients with osteoarthrosis(OA) and from 5 normals. The determinations of IL－1β were carried out using ELISA. The determination of TNF－α was performed by bioassay. Results The concentrations of IL－1β are higher in synovial fluids from OA than those from normal (P＜0.001).TNF－αconcehtrations of IL－1βare low and only 25％of the patients with OA could be ositively detectcd.To further define intra-articular sources of these cytokines,the ability of synoviocytes to produce IL－1βandTNF－αwas examined.Human synoviums isolated from normal(5 cases)and osteoarthritic joints(20 cases)spontaneouslu release Il whencultured in thd presence of serum.The le=vels of IL－1βincrease markedly when synoviocytes were stimulated by IPS either OA or normal,especially in OA.It was mot found that human synoviocyt[1] [2] [3] 下一页
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