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尿路上皮癌模型的制作及BCG对其发生发展的影响 |
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安黎明 江鱼
摘要 目的：研究小鼠尿路上皮癌模型的制作及卡介苗(BCG)对其发生发展的影响。方法：根据化学致癌的“启动——促进”理论，采用N-丁基-N-(4-羟丁基)亚硝胺(BBN)和糖精诱导雄性纯种近交系SSB小鼠发生高度浸润性尿路上皮癌并制作成模型，在此模型基础上进行了BCG对尿路上皮癌发生发展的影响的研究。结果：尿路上皮癌总发生率为24.1%，其中肾盂癌和膀胱癌发生率分别为11.1%和12.9%，未发现输尿管癌。88.9%的肾盂癌和71.4%的膀胱癌为浸润性癌。结论：BCG对尿路上皮癌的发生没有影响，但对其生长有促进作用。
关键词 尿路上皮肿瘤 实验动物模型 卡介苗
Development of mouse model of urothelial carcinoma and BCGs′ influence
AN Li-ming JIANG Yu
(Department of Urology, the Renji Hospital, Shanghai Second Medical University, Shanghai, 200001)
Abstract Purpose:To study the effect of orally administered BCG on the development of mouse urothelial carcinoma caused by chemical carcinogen. Methods: 80 male induced SSB mice were given 0.05% BBN for 8 weeks and 1% saccharin for 12 weeks in drinking water. Then the animals were divided into two groups randomly. One group were given 2.0g/L BCG solution. The experiment was ended at 20th week and all animals were killed and studied pathologically. Results: The total incidence of the urothelium carcinoma was 24.1% and the incidence of the renal pelvis and urinary bladder carcinoma was 16.7% and12.9% respectively. 88.9% of the renal pelvis carcinomas and 71.4% of the urinary blad der carcinomas were invasive. Conclusions: The result indicated that BCG administered orally had no effect on urothelium carcinogenesis, but had a promotion on the growth of urotelium carcinoma.
Key words Urothelial tumor Laboratory animal Bacille Calmette-Guerin
大量动物实验表明，N-丁基-N-(4-羟丁基)亚硝胺〔N-butyl-N-(4-hydroxybutyl)nitrosamine，BBN〕对鼠类膀胱有特异性致癌作用，糖精(saccharin)则对其有促癌作用。同时使用这两种物质诱癌，鲜有其他尿路上皮癌的发生〔1〕。本实验根据化学致癌的“启动——促进”理论(Initiation-promotion theory)，联合使用BBN和糖精成功地诱导了纯种近交系SSB雄性小鼠尿路上皮癌并制作成模型，在此模型上研究了口服卡介苗(BCG)对尿路上皮癌发生发展的影响，现将结果报告如下。
1 材料与方法
1.1 实验动物
选择6周龄纯种近交系SSB雄性小鼠(卫生部上海生物制品研究所医学动物实验中心提供)80只，分笼饲养，每笼5只，笼底垫经过消毒的木屑，每周更换1次。室内温度控制在24℃，湿度控制在60%&plu[1] [2] 下一页
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