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门静脉注射供体脾细胞诱导大鼠移植肾长期存活 |
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庄建平 郭震华 侯健全
摘 要：目的：探讨门静脉内注射供体脾细胞诱导移植肾的免疫耐受情况。方法：实验组Wistar大鼠在肾移植同时将经过预处理的供体SD大鼠脾细胞注入门静脉，对照组则注入生理盐水，然后用环孢素A治疗1周，并以大鼠平均存活时间为标准比较两组结果。结果：对照组平均存活(10.5±2.1)d，实验组为(72.2±32.0)d(P＜0.01)。实验组在肾移植60 d后，再移植SD大鼠和Lewis大鼠的皮肤，发现SD大鼠的皮肤不被排异，Lewis大鼠的皮肤出现排异。结论：门静脉内注射供体脾细胞可诱导肾移植免疫耐受，并且这种耐受具有特异性。
关键词：肾移植 免疫耐受 动物实验
An experimental study on the intraportal injection of
the donor splenocytes on the renal allograft survival time
ZHUANG Jiang-ping
(Department of Urology,Suzhou No.2 People′s Hospital, Suzhou,Jiangsu,215002
GUO Zheng-hua HOU Jian-quan
（Department of Urology,the First Affiliated Hospital of Suzhou Medical College)
Abstract：Purpose：To study on the tolerance of renal allograft.Methods：We built the animal model on the specific tolerance of renal allorgaft.The wistar rats were divided into two groups.While the kidney transplantation was performed the donor rats SD spleen cells which were pretreated were injected into portal vein of the recipient rats wistar in experimental group,0.9% normal solution was injected into portal vein in control group.Results：The median survival peroid of the rats was (10.5±2.1) days in control group,(72.2±32.0) days in experimental group (P＜0.01).60 days after the transplantation,the rats in the experimental group were transplanted with the skin from the SD rats or Lewis rats.Two weeks later,the SD rat′s skin were survived,the Lewis rat′s skin were dead.Conclusions：It was believed that the intraportal injection of donor spleen cells could induce recipients specific tolerance to renal allograft.
Key words：idney transplantation Immune tolerance Animals,laboratory▲
本研究采用供体SD大鼠的脾细胞，经过处理后注入受体Wistar大鼠的门静脉内，同时进行肾移植，然后用环孢素A治疗1周的方法建立成大鼠肾移植免疫耐受的模型。现报告如下。
1 材料与方法
1.1 动物
供体SD大鼠、受体Wistar大鼠由苏州医学院实验动物中心提供，皮肤供体之一Lewis大鼠购自上海实验动物中心。各种大鼠均为近交系，雄性，6～14周，体重200～400 g。
1.2 实验分组及移[1] [2] 下一页
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