|
缺血对兔股动脉平滑肌机能的影响 |
| |
李少华 顾玉东 陈亮 陈德松 李继峰 薛长发
【摘要】目的 研究兔股动脉平滑肌组织中Ca2＋－ATP酶、琥珀酸脱氢酶(SDH)活性和Ca2＋、Mg2＋含量在缺血期间的变化及其与缺血性血管痉挛的关系。方法 在新西兰大白兔的断肢缺血动物模型中，使用组化技术检测股动脉平滑肌组织中Ca2＋－ATP酶、SDH活性的变化，用图像分析系统测定其光密度来代表酶活性；用原子吸收光谱分析法测定股动脉平滑肌组织中Ca2＋、Mg2＋含量的变化。结果 缺血8小时，Ca2＋－ATP酶、SDH活性下降显著(P<0.05),缺血16小时后，Ca2＋－ATP酶、SDH活性下降非常显著(P<0.01),缺血8小时后，Ca2＋含量升高非常显著(P<0.01)。结论 血管平滑肌组织中Ca2＋－ATP酶、SDH活性下降及Ca2＋升高是缺血性血管痉挛的重要原因。 【关键词】再植术 Ca2＋－ATP酶 琥珀酸脱氢酶 钙 血管 痉挛
Effect of Ischemia on Function of Smooth Muscle of Femoral Arteries in Rabbits
LI Shaohua*,GU Yudong, CHEN Liang, et al.
*Department of Orthopaedics,Changhai Hospital, The Second Military Medical University,Shanghai 200433
【Abstract】 Objective To observe the changes of Ca2＋－ATPase and succinate dehydrogenase(SDH) activity and Ca2＋and Mg2＋contents in smooth muscle of femoral arteries in rabbit during ischemia,and to study the relationship between these changes and vasospasm.Methods On the ischemic model of the amputated limbs of New Zealands rabbits,the changs of Ca2＋－ATPase and SDH activity in smooth muscle of femoral arteries were detected with histochemistry method,the image pattern analysis system was used to measure optical density which stood for enzyme activity,the changes of Ca2＋and Mg2＋contents in smooth muscle of femoral arteries were detected with atomic absorption spectroscopy analysis method.Results The Ca 2＋－ATPase and SDH activity significantly decreased at 8 h after ischemia took place(P<0.05), and the decrease became more significantly at 16 h after,ischemia happened(P< 0.01).The content of Ca2＋significantly increased at 8 h after ischemia took place(P< 0.01).Conclusion The decrease of the Ca2＋－ATPase and SDH activity and the increase of Ca2＋content in vascular smooth muscle were important cause of ischemic vasospasm.
【Key words】 Replantation Ca2＋－ATPase Succinate dehydrogenase Calcium Blood vessels Spasm
断肢(指)再植手术，通常认为最佳再植时间为4～6小时，在再植术后，血液循环危象[1] [2] 下一页
上一个医学论文： 眼睑皮肤游离治疗瘢痕性内眦赘皮
下一个医学论文： 脊髓减压术后神经功能恢复机理的实验研究
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 外科论文 >> 正文