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纤溶酶原激活剂及其抑制因子1在大鼠脊髓急性损伤时的表达 |
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王德春 陈中伟 陈峥嵘 张乃娴 宋后燕
【摘要】 目的 观察大鼠脊髓急性损伤时组织型纤溶酶原激活剂（t-PA）和纤溶酶原激活剂抑制因子1（PAI－1）表达的变化，了解t－PA、PAI－1在脊髓急性损伤后所起的作用。方法 35只Wistar大鼠，用改良Allen法制成脊髓中度急性损伤模型，伤后2，6，24，72，168，240小时取材分别进行组织学、免疫组化、图像分析。结果 脊髓中度损伤后，损伤部位脊髓经过了出血、血栓形成、液化、坏死等病理变化。脊髓损伤后3天t-PA表达达到高峰，以后逐渐下降，而PAI－1伤后3天有少量表达，以后逐渐增多。结论 t－PA、PAI－1参与脊髓急性损伤过程，PAI－1对保护残存的运动神经元及胶质细胞具有一定作用。
【关键词】 脊髓损伤 组织学，比较 大鼠 组织型纤溶酶原激活剂
Expression of Tissue-type Plasminogen Activator and Its Inhibitor-1 in Acute Rat Spinal Cord Injuries
WANG Dechun, CHEN Zhongwei, CHEN Zhengrong, et al.
Dept. of Orthopedic Surgery, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032
【Abstract】 Objective To observe the changes of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) expression in acute rat spinal cord injuries (SCI), to learn about the effect of t-PA and PAI-1 on the process of acute spinal cord injury. Methods Using thirty-five Wistar rats, the moderately acute spinal cord injury models were made with modified Allien s methods. Histology examination, immunohistochemistry and map analyses were performed after 2, 6, 24, 72, 168, 240 hours of injury. Results After moderately acute spinal cord injury, pathology changes such as bleeding, thrombosis, liquescence, necrosis, etc occurred in the local area of injured spinal cord. Immunohistochemistry and map analysis demonstrated that the expression of t-PA reached the maximum in 3 days of injury, and subsequently reduced; but only a small amount of PAI-1 was expressed in 3 days of SCI, and then increased gradually. Conclusions t-PA and PAI-1 participate in the process of acute spinal cord injury. PAI-1 has some effects on protecting the remnant motor neurons and gliocytes.
【Key words】 Spinal cord injury Histology, comparative Rats Tissue-type plasminogen activator
脊髓受到外力作用引起急性损伤，除原发性因素如机械作用外，继发性因素也起了非常重要的作用。如细胞外基质成分的改变，细胞因子的作用［1］，氧自由基、一氧化氮、一氧化氮合酶的作用［2］，一系列细[1] [2] 下一页
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