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高氧化还原电位酸性水制备及杀菌效果的试验观察 |
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薄玉霞 张爱荣 马新爱 郭红霞 吉琛
提要 高氧化还原电位酸性水（EOW）是电解加有食盐蒸馏水时，在阳极产生氧化还原电位（ORP）≥1 040 mV，pH值≤3.69，含有效氯<80 mg/L的水。经检测，水中食盐含量为550～950 mg/L时，电解10 min，该水ORP随食盐含量增加而升高；电解8～12 min，ORP随电解时间延长而升高。悬液定量杀菌试验表明，在20℃下，ORP为1 152 mV的该水，对不含蛋白胨悬液中枯草杆菌黑色变种芽胞作用40 min，杀灭率达100％。杀菌效果随ORP升高、温度上升而增强，因蛋白胨存在而减弱。
关键词 高氧化还原电位酸性水 枯草杆菌黑色变种 杀芽胞作用
PREPARATION OF ACIDIC WATER WITH HIGH REDOX POTENTIAL
AND EXPERIMENTAL OBSERVATION ON ITS GERMICIDAL EFFICACY
Bo Yuxia Zhang Airong Ma Xinai
(Henan Provincial Sanitary and Anti-epidemic Station, Zhengzhou 450003)
Guo Hongxia Ji Chen
（Henan Pusheng Industry Co. Ltd.）
Abstract
Acidic water (electrolyzed oxidizing water, EOW) with high redox potential is the water with redox potential (ORP) ≥1040 mV, pH value ≤3.69 and containing available chlorine <80 mg/L, which are produced at the anode during electrolysis of distilled water containing table salt. Examination found that when the table salt content of water was 550-950 mg/L and electrolysis was carried out for 10 min, the ORP of water increased with increasing table salt content. When the eletrolysis time was 8-12 min, the ORP increased with prolonging electrolysis time. The suspension quantitative germicidal test indicated that at 20℃, the killing rate of Bacillus subtilis var. niger spores in suspension without peptone exposed to the water with ORP of 1152 mV attained 100%. The germicidal efficacy increased with increasing ORP and temperature and decreased in presence of peptone.
Key words acidic water with high redox potential Bacillus subtilis var. niger sporicidal effect
高氧化还原电位酸性水（简称EOW）是，在阴、阳两极间有隔膜的电解槽内，电解加入食盐蒸馏水，在阳极产生高氧化还原电位（ORP）、低pH值的水。对其制备条件与杀菌效果进行了试验观察。现将结果报告如下。
1 方法
1.1 高氧化还原电位酸性水的制备
对加食盐量不同的蒸馏水，分别电解不同时间。对制备出的该水，用pHS-25型酸度计的pH复合电极和ORP复合电极分别测pH值及电位值。
1.2 中和剂选择试验
试验菌为枯草杆菌黑色变种（ATCC 9372）芽胞。试验设平行8组：① 消毒剂+菌[1] [2] [3] 下一页
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