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肾癌细胞c |
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许宁 郑学清 石爱平 赵忠文
摘要 目的：阐明c-myc表达在肾癌发生、发展中的作用及临床意义。方法：利用免疫组织化学ABC法结合微波抗原修复和流式细胞技术，对c-myc蛋白在52例肾癌组织及14例癌旁正常肾组织中的表达进行了定性、定量的测定。结果：肾癌组织中c-myc蛋白的阳性率和阳性细胞率分别为76.92%和65.45%±29.19%，高于正常肾组织的14.29%和8.59%±3.66%(P＜0.005)。c-myc在肾癌组织中55.77%分布于胞浆，21.15%分布于胞核，而在正常肾组织则全部分布于胞核。其胞浆表达在病理G2、G3级高于G1级(χ2=9.244，P＜0.005)；临床分期T3、T4期高于T1、T2期(P＜0.001)。结论：c-myc的胞浆表达促进肾癌的发生、发展，并与恶性程度、临床分期有关，是预后不良的重要指征。
关键词 肾肿瘤 免疫组织化学 流式细胞技术 原癌基因蛋白质c-myc类
Cellular localization of c-myc product and clinical
significance in renal cell carcinoma
Xu Ning Zheng Xueqing Shi Aiping et al
(Department of Andrologic Institute,the First Clinical College
Bethune Medical University,Changchun,130027)
Abstract Purpose:To get to know the effect of c-myc protein on the onset and development of renal cell carcinoma and clinical progress.Methods:We have detected expression of c-myc protein qualitatively and quantitatively in 14 cases normal tissue and 52 cases renal cell carcinoma(RCC). Using immunohistochemical technique,and cytoflowmetry and cytoimmunofluorescence.Results:Normal tissue showed staining only of some nuclei while in RCC staining varied from predominantly nuclear to focal or pancytoplasma. Positive rate and positive cell rate of c-myc protein were 76.92% and 65.45%±29.19% respectively in RCC,they were significant higher than those (14.29% and 8.59%±3.66%) of the normal tissue (P＜0.005). Cytoplasmic expression of c-myc was related to high pathological grade (χ2=9.244，P＜0.005) and high clinical stage (P＜0.001).Conclusions:Cytoplasmic expression of c-myc might play important roles in the pathogenisis and was helpful in the evaluation of prognose of patients in RCC.
Key words Kidney neoplasms Immunohistochemistry Flow cytometry Proto-oncogene proteins c-myc
目前国内关于c-myc在肾癌中的研究甚少，而且对c-myc的表达分布及其意义尚有争议。我们利用免疫组织化学(免疫组化)及流式细胞技术对52例肾癌及14例癌旁正常组织中c-myc的表达进行了定性、定量的测定，并就c-myc[1] [2] [3] 下一页
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