一种新的胆胰管穿刺注射法制作重症急性胰腺炎大鼠模型的研究

【摘要】  目的 研究一种新的胆胰管穿刺注射法制作重症急性胰腺炎（severe acute pancreatitis，SAP）大鼠模型。方法 60只Sprague-Dawley大鼠随机分成对照组（30只）和模型组（30只），采用新的胆胰管穿刺逆行胰胆管注射5％牛磺胆酸钠法（简称新的胆胰管注射法）诱导SAP大鼠模型。随后共分1、3、6、12、24 h 5个时间点，动态观察两组大鼠的死亡率、腹水量、血清淀粉酶、胰腺组织病理变化并作病理评分，以及肝、肾、心功能的变化。结果 模型组24 h死亡率为16.67％。各时点，模型组腹水量、血清淀粉酶、谷丙转氨酶、尿素氮、肌酐、心肌型肌酸激酶同工酶值及胰腺组织病理评分均高于对照组（P<0.05），且各项数值呈一稳定的动态变化。结论 这种新的胆胰管穿刺注射法是一种操作简单，病变符合临床特征较为稳定的SAP大鼠模型制作方法。

【关键词】  重症急性胰腺炎；模型，动物；胆胰管；穿刺；大鼠

The research of a new method of puncturing biliopancreatic duct injection on rat model with severe acute pancreatitis
　　
　　ZHANG Zhongwei， ZHANG Qiyu，ZHOU Mengtao， et al.
 
　　Department of General Surgery， the Second Hospital of Shaoxing， Shaoxing， Zhejiang 312000

　　Abstract  Objective  To study a new method of puncturing biliopancreatic duct injection on rat model with severe acute pancreatitis (SAP). Methods  Sixty SD rats were randomly divided into two groups： the SAP group （n=30） and the control group （n=30）. The SAP model was induced by the new method of retrograde injection of 5% sodium taurocholate into biliopancreatic duct after biliopancreatic duct punctured (to be abbreviated as the new method of puncturing biliopancreatic duct injection). All animals were sacrificed at 1， 3， 6， 12， 24 hours， respectively， after the onset of induction. Then rat mortality， ascites volume， serum amylase and function of liver， kidney， heart were examined in each group. Histological change of pancreas was also evaluated. Results  The mortality of SAP group at 24 hours was 16.67%. Levels of ascites volume， serum amylase (AMY)， alanine aminotransferase (ALT) ， blood urea nitrogen (BUN)， MB isoenzyme of creatine kinase (CKMB) and histological scoring in SAP group were all significantly higher than those of the control group at each time point （P<0.05). And data were in stabile kinetic variance. Conclusion  The new method of puncturing biliopancreati

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