【摘要】 目的 观察黄体酮对脑外伤后脑组织内炎症反应信号转导因子核因子κB(NFκB)、炎症细胞因子如肿瘤坏死因子(TNFα)和白介素1β(IL1β)水平的影响，探讨其对创伤性脑外伤(TBI)继发性脑损伤的保护作用及可能的作用机制。方法 采用Freeney法造成大鼠脑挫裂伤模型，在伤后1、3、5、7天不同时相点用ELISA法、免疫组化法分别检测4组大鼠脑组织中NFκB、TNFα、IL1β水平的变化。结果 伤后注射黄体酮治疗组脑组织NFκB、TNFα、IL1β 3种炎性因子水平均比脑创伤组低(P<0.05)。结论 注射黄体酮能降低脑外伤后炎性信号因子表达，减轻脑外伤后脑组织的炎症反应，对脑组织起一定的保护作用。
【关键词】 颅脑损伤;核因子κB;肿瘤坏死因子;白介素1β;炎症;黄体酮
Effect of progesterone administration on expression of the inflammatory factors after brain injury in rats
SHEN Bin,HANG Chunhua
(Department of Neurosurgery,Liuhe People s Hospital,Nanjing 211500,China)
Abstract： Objective To observe the influence of progesterone administration on expression of the inflammatory factors such as nuclear factorkappa B(NFκB),tumor necrosis factorα (TNFα) and interleukin1β(IL1β) following traumatic brain injury and to explore the possible mechanisms for its neuroprotective effects.Methods Parietal brain contusion was adopted using Freeney s weightdropping method.The levels of NFκB,TNFα and IL1β expression in brain tissues were detected by ELISA or immunohistochemistry at each corresponding time point.Results The result showed a persistent upregulation of NFκB,TNFα,IL1β in the area surrounding the injured brain.The level of inflammatory factors expression and the number of neurocyte apoptosis can be decreased by injections of progesterone(P<0.05).Conclusion Progesterone administration can down-regulate the expression of the inflammatory factors,alleviate the inflammatory response after TBI and protect brain tissues effectively.
Key words:brain injury;inflammatory factors;nuclear factorkappa B;tumor necrosis factorα;interleukin1β;progesterone
近年来，创伤性脑外伤(TBI)的发生率不断增高，成为45岁以下个体意外死亡的首要原因[1]。如何减轻TBI的继发性脑损伤成为近年来科学家努力研究的方向。目前普遍认为，TBI后脑组织炎症反应是导致继发性脑损伤的一个重要因素，并且证实炎性信号因子转导因子核因子κB(NFκB)、炎症细胞因子肿瘤坏死因子α(TNFα)、白介素1β(IL1β)等加重了TBI后继发性脑损伤。作为神经甾体的一员，黄体酮广泛存在于神经系统。已有研究报道[2]动物TBI后，注射黄体酮有抑制脑组织炎症
[1] [2] [3] [4] [5] 下一页
您现在的位置： 绿色健康网 >> 医学论文 >> 外科论文 >> 正文