【摘要】 目的 检测异丙酚对脑缺血-再灌注后大鼠血清、脑组织氧化水平的影响。方法 采用大脑中动脉线栓法制备大鼠脑缺血再灌注模型，随机分为假手术组、缺血再灌注模型组和异丙酚治疗组(100mg/kg)，缺血再灌注24小时后检测大鼠血清、脑组织匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHpx)、丙二醛(MDA)和一氧化氮(NO)的活性。结果 与模型组比较，异丙酚可以明显提高缺血-再灌注大鼠血清和脑组织中SOD、GSHpx活性，降低MDA、NO含量(P<0.05,P<0.01)。结论 异丙酚可通过提高脑缺血-再灌注后大鼠血清、脑组织的抗氧化能力，减少脂质过氧化和NO生成而发挥对脑缺血-再灌注时的保护作用。
【关键词】 异丙酚;缺血再灌注;抗氧化;脂质过氧化;一氧化碳
Influence of propofol on oxidation level in cerebral ischemia reperfusion rats in vivo
LIU Xianming,LI Guoping,LIU Yuefen
(Department of Neurosurgery,Municipal Hospital of Qingdao,Qingdao 266071,China)
Abstract: Objective To study influences of propofol on serum and brain tissue oxidation level in cerebral ischemia reperfusion rats in vivo.Methods The cerebral ischemia reperfusion rats models were made using thread lock on middle cerebral artery method and then randomly divided into three groups: sham operation group,cerebral ischemia reperfusion model group and propofol treatment group(100mg/kg).After 24 hours of ischemia reperfusion,the activities of superoxide dismutase(SOD),glutathione peroxidase(GSHpx) and the contents of malondialdehyde(MDA),nitricoxide (NO) in serum and in brain tissue homogenates were detected.Results Compared with the model group,propofol significantly increased the activities of SOD and GSHpx and decreased the contents of MDA and NO in serum and brain tissue homogenates of cerebral ischemia reperfusion rats(P<0.05,P<0.01).Conclusion Propofol could protect the brain from cerebral ischemia reperfusion injury through its activities of improving the antioxidant capability and decreasing lipid peroxidation and NO formation.
Key words:propofol;ischemia reperfusion;antioxidant;lipid peroxidation;NO
脑缺血后大量的自由基生成，引起脑内脂质过氧化作用加强，导致细胞膜、线粒体膜及血脑屏障损害，引起和加重缺血和脑组织水肿，同时自由基及脂质过氧化产物可以影响多种抗氧化酶类的活性[1，2]。脑组织对自由基的损伤极为敏感，在缺血性脑损伤中，缺血过程中固然可产生大量的自由基，但大量研究表明再灌注期间生成更多。在缺血性脑损伤中自由基反应启动于缺血期，但明显过氧化反应却发生在再灌注期[3，4]。静脉麻醉药异丙酚的化学结构与酚基类自由基清除剂相类似，其
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