【摘要】 目的 通过研究早期高压氧治疗对大鼠颅脑损伤后相关基因Bcl-2、Bax表达影响,探讨其对损伤神经细胞的早期保护作用。方法 清洁级SD大鼠100只 ，随机分为正常组(Nor)20只、创伤组(TBI)40只、高压氧治疗组(HBOT)40只。采用Allen′s改良法建立大鼠自由落体脑损伤模型，在损伤6 h内予以高压氧治疗。伤后8 h及1、3、5、8天处死动物取材，苏木精-伊红染色观察细胞形态学变化，流式细胞仪检测各观察点细胞凋亡率，免疫组织化学染色方法检测皮质及海马细胞中Bcl-2、Bax蛋白的表达。结果 HBOT组各时点皮质及海马细胞均较TBI组形态规则、排列整齐，而且层次较多，胞核浓染、固缩的现象明显减少。与TBI组相比，HBOT组各时点的细胞凋亡率均显著下降(P<0.05)。HBOT组各时点Bcl-2表达强度均较TBI组增强(P<0.05，P<0.01),Bax表达强度均较TBI组显著降低(P<0.01)。结论 早期高压氧治疗能明显促进颅脑损伤后Bcl-2的高表达，抑制Bax的表达，能有效抑制颅脑损伤后细胞凋亡。
【关键词】 脑损伤;早期;高压氧;Bcl-2;Bax;细胞凋亡
The effect of early hyperbaric oxygenation treatment on the neurocyte
apoptosis following craniocerebral injury
LIU Yong， CHEN Xianglin, ZHU Yufu, YAN Jingfeng, KONG Lingsheng, FENG Jun, HU Xiaobing
(Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China)
Abstract: Objective To investigate the effects of early hyperbaric oxygenation treatment (HBOT) on the expression of apoptosis genes Bcl-2 and Bax following the rat brain injury and study the neuronal protecting mechanism. Methods 100 Sprague-Dawley rats were divided into 3 groups: Normal group (20 rats), TBI group (40 rats), HBOT group (40 rats). Based upon the Feeney′s model of modified Allen′s method, experimental animals were treated with HBO within 6 hours. The animals were sacrificed at the 8th hour, and on days 1, 3, 5 and 8, respectively. Their hippocampus cells were observed for morphological changes by H-E staining; the apoptosis rate of injured hippocampus cortex was detected by flow cytometry; and the expression of protein Bcl-2 and Bax was detected with immunohistochemical staining. Results H-E staining indicated that, in contrast to TBI group, in HBOT group the hippocampus cortex and the hippocampus cells at appropriate times had regular morphology, orderly arrangement and more stratal distributions, with a significant reduction in nuclear dense-staining and pyknosis, and marked dec
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