【摘要】 目的 探讨移植肾慢性排斥反应(CR)时转化生长因子-β1诱导的抗凋亡因子-1(TIAF-1)的表达及其意义。方法 采用SP免疫组织化学染色法对6例因肾细胞癌行肾切除的未受肿瘤侵犯的正常肾组织和8例供肾正常肾组织、16例急性排斥反应和28例慢性排斥反应移植肾组织中TIAF-1的表达进行观察，并对间质浸润细胞中CD3、CD20、CD68阳性细胞数进行分析。结果 正常供肾组织未见TIAF-1表达。急性排斥反应移植肾间质TIAF-1阳性细胞数明显增加，肾小管上皮细胞呈微弱的TIAF-1表达。在慢性排斥反应移植肾组织中，TIAF-1在间质浸润细胞中的表达呈弥漫性、强阳性,较多的肾小管上皮细胞呈高度染色。移植肾间质浸润的TIAF-1阳性细胞数与CD3和CD20阳性细胞数呈正相关。结论 TIAF-1在慢性排斥反应移植肾组织中的表达可能与Th1细胞向Th2细胞的免疫偏离有关，移植肾肾小管上皮细胞的TIAF-1表达可能反映了某种保护性机制的诱导。
【关键词】 肾移植;慢性排斥反应;转化生长因子-β1诱导的抗凋亡因子-1
The expression of TIAF-1 in chronic kidney allograft rejection and its significance
ZHOU Guangchen1, HE Xiaozhou2, CHAO Zhifu2
(1.Department of Urologic Surgery, The Medical College of Yangzhou University, Yangzhou, Jiangsu 225001, China;
2.Department of Urologic Surgery, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213000)
Abstract: Objective To investigate the characteristics of TGF-β1-induced antiapoptotic factor-1 (TIAF-1) expression in renal allograft of chronic rejection (CR) and to study the possible pathogenic role of TIAF-1 in chronic allograft rejection. Methods Immunohistochemistry of streptavidin-perosidase (SP) method was applied in the observation of the expression of TIAF-1 in each group, including tissues obtained from the unaffected part of nephrectomized kidneys with renal cell carcinoma (n=6) and cadaveric donor of normal kidneys (n=8) as the control group, tissues with acute rejection (AR, n=16) and those with chronic rejection (CR, n=28), and in the analysis combined with the number of positive cells of CD3, CD20 and CD68 infiltrated in the interstitium. Results The expression of TIAF-1 was not detectable in normal kidney. The expression of TIAF-1 positive cells in the interstitium of renal allografts with acute rejection was up-regulated and TIAF-1 was weakly expressed in tubular epithelial cells. In tissues with chronic renal allograft rejection, a high and diffusive expression of TIAF-1 was found
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