【摘要】 目的：观察急性肾功能衰竭(ARF)家兔血清一氧化氮(NO)及其合酶的变化，探讨NO在ARF发病机制中的作用。方法：家兔60只均分为四组(n=15)。ARF模型Ⅰ组：皮下注射1%HgCl2(1.3ml/kg.bw);ARF模型Ⅱ组：肌肉注射50%甘油(10ml/kg.bw);以等量生理盐水代替HgCl2和甘油作为对照Ⅰ、Ⅱ组。24h后，所有动物颈总动脉放血备检，制备肾匀浆。经Aeroset型全自动生化分析仪测定血清BUN、SCr水平，以硝酸还原酶法、化学显色法分别检测血清及肾组织匀浆NO含量及NOS、iNOS活性，肾组织匀浆总蛋白以双缩脲法定量。结果：ARF模型Ⅰ、Ⅱ组血清NO含量及NOS、iNOS活性分别高于相应对照Ⅰ、Ⅱ组(P<0.05～0.01)，肾匀浆NO含量显著高于相应对照组(P<0.01)。结论： NO在ARF发病过程中发挥保护及损伤的双重作用。
【关键词】 急性肾功能衰竭;一氧化氮;一氧化氮合酶
Changes of Nitric Oxide and Nitric Oxide Synthase in Rabbits with Acute Renal Failure
MIAO Wen-long, ZHAO Yong-quan, HOU Ya-li, et al.
The First Affiliated Hospital, Hebei North University, Zhangjiakou, 075000, China
【ABSTRACT】 Objective： To observe the changes of nitric oxide (NO) and nitric oxide synthase (NOS) in rabbits with acute renal failure(ARF), and inquire into the effects of NO in the pathogenesis of ARF.Methods：Sixty rabbits were divided into four groups (averaging 15 rabbits in each group). The ARF model group I was copied by hypodermic injection 1% HgCl2 of 1.3ml/kg, and the ARF mode l group II was copied by intramuscular injection 50% glycerinum of 10 ml/kg. The control group I and Ⅱ were injected by equivalent volume of normal saline. After 24 hours, all rabbits were cannulated to facilitate blood with drawal for serum sample, and making renal homogenate. The BUN and SCr content in serum were determined by Aerosetautomatic biochemical analyzer. And the NO, NOS and inducible nitric oxidesynthase(iNOS) in blood serum and renal homogenate were determined by nitrate reductase method and chemical chromogenic reaction. The total protein(TP) of renal homogenate was determined by biuret reaction. Results：It showed that NO, NOS and iNOS in serum of ARF groupI and II were significantly increased compared with control group Ⅰ and Ⅱ (P<0.05～0.01). The NO content in renal homogenate of ARF group I and II was significantly increased compared with control group Ⅰ and Ⅱ (P<0.01). Conclusio
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