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不同转染方法对逆转录病毒转染效率影响的研究 |
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p;culture medium.4，adding virus supernatant firstly，complementing culture medium after 6 hours，quitting medium after culturing overnight，and then adding virus supernatant，culture medium was complemented after 6 hours.5，the classical transfected method：adding virus supernatant and culture medium on the same time.To observe fluorescence color with immunofluorescence microscope after transfecting 2 days.Testing the NO content of each group′s culture medium after transfecting stably about 4 weeks，and detecting expression of hIL1Ra in supemate by ELISA.Results Enzyme digestion identificated the recombination was correct.To observe green fluorescence with immunofluorescence microscope.Among the NO content from 1 to 5 group，the highest is 4 group and the lest is 5 group，There is statistical significance in comparison between any two means.Among the hIL1Ra content from 1 to 5 group，the highest is 4 group and the lest is 5 group.There is statistical significance in comparison between any two means.Conclusion Four adding methods′transfecting efficiency was higher than classical method.
Key words：transfect methods；retrovirus；transfecting efficiency
随着基因工程的发展，基因转染越来越多地应用到临床和科研领域。但是如何上一页 [1] [2] [3] [4] [5] 下一页
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