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血管紧张素原基因M235T分子变异与2型糖尿病肾病的关系 |
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王健军 朱席琳 杨凌 周文郁 李红兵 付汉箐 朱良湘 袁申元
【摘要】 目的 探讨血管紧张素原(angiotensinogen,AGT)基因M235T分子变异与中国人无肾病并发症的2型糖尿病(diabetes mellitus,DM)、2型糖尿病肾病(diabetic nephropathy,DN)的关系。方法 用PCR及RFLP方法对84例DM、96例DN及98名正常对照进行了AGT基因M235T多态性的检测。结果 DN组T等位基因频率0.82，TT基因型频率0.70，与对照组(0.63，0.43)比较有显著差异(P＝0.003，P＝0.0004)；校正了DN的几种危险因素后，TT基因型对DN的OR为3.47(95%CI为1.51～7.94，P＝0.0033)。DM组基因型频率分布与对照组比较无显著差异(P＞0.05)。结论 AGT基因TT型可能是中国人群2型糖尿病肾病的独立危险因素之一。
【关键词】 血管紧张素原 基因 糖尿病肾病 2型糖尿病 多聚酶链式反应
Relationships of angiotensinogen gene M235T variant with diabetic nephropathy in Chinese type 2 diabetes mellitus
WANG Jianjun*, ZHU Xilin, YANG Ling, ZHOU Wenyu, LI Hongbing, FU Hanqin, ZHU Liangxiang, YUAN Shenyuan.*Department of Endocrinology, Affiliated Beijing Tongren Hospital, Capital University of Medical Sciences,Beijing 100730 P.R.China. E-mail:Sunwj@public 3.bta.net.cn
【Abstract】 Objective To investigate whether angiotensinogen (AGT) gene M235T variant is associated with type 2 diabetes mellitus without nephropathy(DM) and/or diabetic nephropathy(DN) in Chinese type 2 diabetes.Methods AGT genotypes of 84 cases with DM, 96 with DN and 98 controls were determined by polymerase chain reaction(PCR) amplification and restriction fragment length polymorphism analysis of the region of the variant.Results Increased frequencies of T allele(0.82) and TT genotype (0.70) were observed in 96 subjects with DN, compared with those observed in 98 control subjects (0.63 and 0.43 respectively,P=0.003,P=0.0004).The odds ratio associated with TT genotype was 3.47(95% CI 1.51-7.94,P=0.0033) for DN in analysis adjusted for several DN risk factors. There was no difference in allele distribution between 84 DM patients and the contorls.Conclusion TT genotype of the AGT gene might be an independent risk factor of diabetic nephropathy in Chinese patients with type 2 diabetes mellitus.
【Key words】 Angiotensinogen Gene Diabetic nephropathy Type 2 diabe[1] [2] [3] [4] [5] [6] 下一页
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