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人脑肿瘤猴病毒40的感染状况及来源研究 |
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步星耀 章翔 张旭 易声禹
摘 要：目的 探讨人脑肿瘤猴病毒40(SV40)的感染状况及来源。方法 采用聚合酶链反应(PCR)和斑点杂交方法，检测人脑肿瘤组织516份，健康人外周血80份，精液50份，人胚胎组织100份，正常人脑组织30份以及SHG44和BT32 5两株人脑胶质瘤细胞系中SV40的DNA序列。结果人脑肿瘤组织SV40 DNA阳性率为36.4%(188/516)；健康人外周血16.3%(13/80)；精液22.0%(11/50)；人胚胎组织8.0%(8/100)；正常人脑组织6.7%(2/30)。SHG44及BT325两株细胞中也分别检测出SV40的DNA序列。人脑肿瘤组织、健康人外周血及精液与健康人脑组织及人胚胎组织阳性率相比，差异有显著性(P＜0.05)。结论 ①人脑肿瘤有较高的SV40感染率；②SV40与人脑肿瘤的发生有密切关系；③SV40可能通过水平和垂直两种途径在人群中传播。
关键词：脑肿瘤；猴病毒40；聚合酶链反应；斑点杂交
A study of simian virus 40 infection and its origin in human brain tumors
BU Xingyao
(Department of Neurosurgery, Henan Provincial People's Hospital,Zhengzhou 450003,China)
ZHANG Xiang ZHANG Xu
Abstract：Objective To study the status of simian virus 40 (SV40) infection and its origin in human brain tumors. Methods Polymerase chain reaction (PCR) and Dot blot hybridization were used to detect SV40 DNA sequences in 516 human brain tumor tissues, 80 peripheral blood cells and 50 sperm fluids from healthy individuals, 100 human embryo tissues from artificial abortion,30 normal brain tissues and two human glioma cell lines: SHG44 and BT325. Results SV40 DNA sequences were found in 36.4% of human brain tumors (188/516),16.3% of healthy peripheral blood cells (13/80),22.0% of healthy semen (11/50), 8.0% of human embry otissues (8/100) and 6.7% of normal brain tissues (2/30). SV40 DNA sequences were also detected in SHG44 and BT325 cell lines. The positive rates of SV40 DNA in human brain tumors, peripheral blood cells and semen from healthy donors were sign ificantly higher than those in human normal brain and embryo tissues (P＜0 .05). Conclusions ①SV40 has a higher infection rate in human brain tumors,②SV40 is closely related to the etiopathogenesis of human brain tumors,③the ways of SV40 spread may due to both horizontal and diaplacenta linfections in human population.
Key words：Brain tumors;Simian virus 40;Ploymerase cha in reaction;Dot blot hybridization▲[1] [2] 下一页
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