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铅中毒对小鼠颌下腺神经生长因子基因表达的影响 |
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丁斐 陆璐 曹铮 仲齐庆 顾晓松
摘要 目的 探讨铅中毒对小鼠颌下腺的毒性作用及对神经生长因子基因(NGF mRNA)表达的影响。方法 采用小鼠实验性铅中毒模型，通过光镜与电镜观察颌下腺的病理组织学变化。应用地高辛标记的人NGF DNA探针，采用原位杂交的方法，定量分析铅对小鼠颌下腺NGF mRNA表达的影响。结果 实验性铅中毒小鼠体重下降，血铅浓度升高，颌下腺铅含量增加。光镜和电镜结果呈现铅中毒小鼠颌下腺腺叶萎缩，纤维增生，腺间质血管扩张，腺间隙增加。粗面内质网扩张，线粒体肿胀。图像分析结果表明，铅中毒小鼠颌下腺纹状管和颗粒曲管直径减小。原位杂交的结果显示，铅中毒小鼠颌下腺颗粒曲管和纹状管的杂交信号显著减弱，颌下腺组织中NGF mRNA表达减少。结论 铅对小鼠颌下腺具有毒性作用，影响颌下腺NGF的基因表达。
关键词 铅中毒 颌下腺 神经生长因子 基因表达
The Effects of Lead Poisoning on Expression of Nerve Growth Factor Gene of Submandibular Gland in Mice
DING Fei*, LU Lu, CAO Zheng, et al.
*Department of Hygienic Toxicology, Nantong Medical College, Nantong City, Jiangsu, 226001
Abstract Objective To study the toxicity of lead poisoning to submandibular gland and its effects on nerve growth factor (NGF) gene expression in mice. Methods An experimental model with lead poisoning was established and its histopathological changes in the submandibular gland of mice were observed under light and electronic microscopes. Effects of lead poisoning on expression of NGF mRNA in submandibular gland were analyzed quantitatively by human NGF DNA probe labelled with digoxin with in situ hybridization. Results Body weight of the mice with experimental lead poisoning reduced, and their lead levels in blood and submandibular gland increased. Lobular atrophy, fibrous hypertrophy, angiectasis of the stroma and enlargement of lobule interstitial in mouse submandibular gland with lead poisoning could be found under light and electron microscopes. Their rough surfaced endoplasmic reticulum was extended and mitochondrion swollen. Graphic analysis showed that diameters of the secretory striate ducts and granular tubules decreased in the mice with lead poisoning. Results of in situ hybridization indicated that hybridized signals in the granular and secretory striate ducts and granular tubules reduced significantly, and NGF mRNA expression decreased. Conclusion Lead is toxic to the sub[1] [2] 下一页
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