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禁食对大鼠肝脏胰岛素样生长因子 |
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李启富 Bernadette Brat Paul Czernichow
摘要 目的 探讨营养状态调节机体生长发育的分子生物学机制。方法 利用 Northern印迹技术，分析禁食对雄性Wistar大鼠肝胰岛素样生长因子-Ⅰ（IGF-Ⅰ）和胰岛素样生长因子结合蛋白（IGFBP-1）基因表达的影响。结果 禁食48小时后大鼠体重下降11.8%、血糖下降50.8%；禁食48小时后，肝IGF-Ⅰ mRNA含量下降32.7%；与此相反，禁食后肝IGFBP-1 mRNA 含量逐渐上升，禁食24小时后IGFBP-1 mRNA含量为正常的2.5倍；禁食后肝白蛋白mRNA含量无明显变化。结论 禁食抑制大鼠肝IGF-Ⅰ基因表达，促进IGFBP-1基因表达。
关键词 禁食 胰岛素样生长因子Ⅰ 胰岛素样生长因子结合蛋白质1 基因表达
Effects of Fasting on Expression of Insulin-like Growth Factor-Ⅰ and IGF Binding Protein-1 Genes in the Liver of Rats
LI Qifu*, Bernadette Brat , Paul Czernichow.
*Department of Endocrinology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016
Abstract Objective To investigate the molecular mechanisms of body development and growth regulated by nutritional status. Methods Effects of fasting on the expression of insulin-like growth factor-Ⅰ （IGF-Ⅰ）and IGF binding protein-1 （IGFBP-1）genes were analyzed by Northern blotting technique in rats. Results Fasting for 48 hours, body weight of the rats decreased by 11.8%, level of blood glucose by 50.8% and content of IGF-Ⅰ mRNA in the liver by 32.7%（P<0.05）. On the contrary, content of IGFBP-1 mRNA in the liver of fasted rats increased gradually, with a 2.5 time increase after 24 h fasting (P<0.05). There was no significant change in the level of liver albumin mRNA after fasting. Conclusion Fasting could inhibit the expression of IGF-Ⅰ gene and enhance the expression of IGFBP-1 gene.
Key words Fasting Insulin-like growth factor Ⅰ Insulin-like growth-factor binding protein 1 Gene expression
遗传、激素和营养三方面因素相互作用，调节人和动物的生长发育。研究表明，生长激素的促生长作用主要由胰岛素样生长因子-Ⅰ(insulin-like growth factor-Ⅰ, IGF-Ⅰ)介导。IGF-Ⅰ由70个氨基酸残基组成，由生长激素作用于靶组织（以肝为主）而合成产生，它参与多种细胞的分化增殖［1］。 胰岛素样生长因子结合蛋白(IGF binding protein, IGFBP)通过与IGF-Ⅰ特异结合，调节IGF的生物学作用。
营养不良，比如蛋白质或热量供给不足，可致生长发育迟缓。但其分子生物学机制尚不十分清楚。一些研究表明，IGF-Ⅰ和IGFBP的合成和分泌，均受到机体营养状态的影响［2］。
为探讨营养状态调节机体生长发育的分子生物学机制，我们观察了禁食对大鼠[1] [2] 下一页
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