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中国人胃癌组织微卫星DNA的不稳定性研究 |
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王永信 柯杨 宁涛 冯丽雅 路桂荣 刘伟莉 鄂征
【摘要】 目的 为探明中国人胃癌的微卫星DNA的不稳定性(microsatellite instability, MSI)频率。方法 选择了29个多态微卫星标记，采用PCR-聚丙烯酰胺凝胶电泳及银染技术对42例胃癌组织进行了MSI分析。 结果 中国人胃癌，在29个位点上平均 MSI频率为33.9%。在D3S1067、D3S1577、D8S279、D9S257、D1S248、D7S520及D2S147等位点上存在高频率的MSI，其中D3S1577和D3S1067(51.35%)MSI发生频率最高。胃癌的不同病理类型表现出不同的MSI。低分化胃癌和印戒细胞癌与高分化癌比，MSI频率明显增高(P＜0.01)；而低分化胃癌和印戒细胞癌之间MSI无显著差异(P＞0.05)。结论 MSI在中国人胃癌中起着重要作用，尤其在低分化胃癌中。这些资料进一步证明，高低分化的胃癌可能具有不同的发病机理。
【关键词】 胃癌 微卫星DNA 微卫星不稳定性
STUDIES OF MICROSATELLITE INSTABILITY IN CHINESE GASTRIC CANCER TISSUES Wang Yongxin*, Ke Yang, Ning Tao, Feng Liya, Lu Guirong, Liu Weili, E Zheng.* Department of Cancer Genetics, Beijing Institute for Cancer Research, Beijing Medical University, Beijing 100034,P.R.China
【Abstract】 Objective To identify the microsatellite instability (MSI) rates in Chinese gastric cancer samples. Methods 29 microsatellite markers were selected to examine 42 paired gastric cancer tissues for MSI on all chromosomes except Y. Results The total frequency of MSI in all 42 gastric cancers was 33.9% with higher rates at loci of D3S1577, D3S1067, D8S279, D9S257, D1S248, D7S520 and D2S147, and the highest rate at D3S1577 and D3S1067(51.35%). MSI varied with different pathological types. The frequencies of MSI were significantly higher in poorly differentiated tumors and signet cell types, compared with well differentiated tumors (P＝0.0026 and 0.0013 by chi-square test), and no difference was noted between poorly differentiated and signet cell types. Conclusion MSI may play an important role in Chinese gastric cancer, particularly the poorly differentiated adenocarcinomas. The data presented here further support the previous hypothesis that pathologically distinct subtypes of gastric cancer undergo different genetic pathways during tumorigenesis.
【Key words】 Gastric cancer Microsatellite DNA Microsatellite instability
胃癌的发生、发展是一个多基因变异累积的复杂过程，如癌基因、抑癌基因、错配修复基因等异常。70年代末，人[1] [2] 下一页
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