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营养不良新生大鼠胰岛素与胰高血糖素及IGF |
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李启富 Bernadette Brat Paul Czernichow
【摘要】 目的 探讨营养不良对胚胎胰岛细胞功能的影响。方法 限制妊娠晚期(14～21天)雌性大鼠摄食量(低于正常50%)以制备营养不良新生大鼠模型，利用Northern印迹法分析胰腺胰岛素、胰高血糖素的基因表达，以及胰腺和肝脏胰岛素样生长因子-Ⅱ(IGF-Ⅱ)基因表达。结果 营养不良新生大鼠胰腺胰岛素mRNA含量明显低于正常对照组(P<0.05)，而胰高血糖素mRNA含量无明显变化；营养不良新生大鼠胰腺和肝脏IGF-ⅡmRNA含量与正常对照比较无明显变化。结论 胚胎期营养不良可抑制胰岛素基因表达，而对胰高血糖素和IGF-Ⅱ基因表达影响不明显。
【关键词】 营养障碍 胰岛素 胰高血糖素 胰岛素样生长因子Ⅱ 基因表达
Changes in Expression of Genes for Insulin, Glucagon and
IGF-II in Neonatal Rats with Malnutrition
LI Qifu*, Bernadette Brat, Paul Czernichow.
*Department of Endocrinology, The First Affiliated Hospital,
Chongqing Medical University, Chongqing 400016
【Abstract】 Objective To study the effects of malnutrition on the function of cells in fetal islet of pancreas. Methods A neonatal rat model with malnutrition was prepared by maternal food restriction （fed with less than half amount of the normal）during the 14th to 21st days of pregnancy. Expression of genes for insulin and glucagon in the pancreas, as well as expression of genes for insulin-like growth factor II （IGF-II）in the pancreas and liver of the rats were analyzed with Northern blotting technique. Results Pancreatic content of insulin mRNA was significantly lower in neonatal rats with malnutrition than that in the normal group（P<0.05）, but no obvious changes in glucagon mRNA in the pancreas was found. There was no significant difference in IGF-II mRNA content in the pancreas and liver between the neonatal rats with malnutrition and the normal control group. Conclusion Malnutrition during rat fetal development could cause inhibition of expression of insulin gene, but no obvious effects on the expression of glucagon and IGF-II genes.
【Key words】 Nutrition desorders Insulin Glucagon Insulin-like growth factor-Ⅱ Gene expression
流行病学研究显示，胎儿出生时体重与其成年期糖耐量有显著相关关系；即低体重胎儿成年后更容易发生糖耐量异常、Ⅱ型糖尿病和胰岛素抵抗综合征(X综合征)［1］。提示胎儿期营养供给不足，可能导致胎儿胰岛的发育和功能发生不可逆损伤，从而引发[1] [2] 下一页
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