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血清学标志阴性的非甲 戊型肝炎的病原学研究 |
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王佑春 庄辉 张华远 R Ling 李倬 毛群颍 吴晓音 TJ Harrison 李河民
【 摘要 】 目的 对血清学标志阴性的非甲～戊型肝炎进行病原学研究。 方法 用HBV PCR、HCV RT-PCR和HEV RT-PCR分别检测血清学标志阴性的非甲～戊型肝炎患者血清，并对其部分阳性产物进行克隆测序。 结果 87例非甲～戊型肝炎血清HBV DNA均为阴性，9例(10.3%)为HCV RNA 阳性,部分经测序证实为HCV 1b亚型；余78例为HBV DNA和HCV RNA均阴性。该78例中，14例因无血清未作HEV RNA检测，余64例中49例(76.6%)为HEV RNA阴性，15例(23.4%)为HEV RNA 阳性。经序列分析显示，其中9例为典型的中国HEV株基因序列，6例变异较大，与典型的中国株基因序列的同源性仅为80％左右。49例HBV DNA、HCV RNA 和HEV RNA均阴性的血清中16例(32.6%)HGV RNA阳性。由此可见，该87例中至少有9例为HCV感染，15例为HEV感染，16例为HGV感染。 结论 对血清学标志阴性的非甲～戊型肝炎的病人应该用PCR法进行病原学分型，以明确其诊断。
【 主题词 】 肝炎病毒，庚型 肝炎病毒，乙型 肝炎病毒，丙型 肝炎病毒，戊型 非甲～戊型肝炎
Studies on the etiology of patients with serologically negative non A～E hepatitis WANG Youchun,ZHUANG Hui,ZHANG Huayuan, et al.National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050
【 Abstract 】 Objective To study the etiology of serologically negative non A～E hepatitis . Methods The sera from serologically negative non A～E hepatitis patients were tested for HBV DNA,HCV RNA, HEV RNA and HGV RNA with PCR method and some PCR positive products were cloned and sequenced. Results Nine of them (10.3%) were positive for HCV RNA. 64 sera with negative HBV DNA and HCV RNA were tested for HEV RNA.15 of them(23.4%)were HEV RNA positive. Nine of the 15 HEV sequences have 95% homology and the remaining 6 have only 80% homology as compared with the Chinese HEV strain at the nucleotide level. Of 49 sera negative for HBV DNA,HCV RNA and HEV RNA, 16 were HGV RNA positive (32.6%) by RT-nPCR suggesting that the patients were infected with HGV. Conclusion The data suggest that the patients with serologically negative non A～E hepatitis have a different etiology and should be tested by PCR to identify the disease.
【 Subject words 】 Hepatitis B virus Hepatitis C virus Hepatitis E virus Hepatitis G virus non-A-E hepatitis
国内外文献报道，在急性散发性病毒性肝炎中，非甲～戊型肝炎约有(10～20)％。为探讨非甲～戊型肝炎的病原，我们对87例血清学标志阴性的非甲～戊型肝炎进行了病原学研究，现将结果报告[1] [2] 下一页
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