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细胞间旁分泌在牵张刺激心肌细胞肥大中的作用 |
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冯兵 王德文 何作云
［摘 要］ 目的： 探讨细胞间旁分泌在牵张刺激心肌细胞肥大中的作用。方法： 用牵张刺激培养成纤维细胞、微血管内皮细胞的条件培养基，刺激心肌细胞，观察［3H］-Leu掺入的变化。 结果： 牵张培养成纤维细胞、微血管内皮细胞的条件培养基均可显著刺激心肌细胞［3H］-Leu掺入。条件培养基中血管紧张素II、内皮素含量显著升高。用特异的血管紧张素II、内皮素受体拮抗剂可明显抑制心肌细胞［3H］-Leu掺入率，联合应用时抑制率可达80%以上。结论： 牵张刺激可通过诱导心肌间质细胞内分泌活化启动心肌肥大反应。
［主题词］ 物理刺激； 成纤维细胞； 内皮，血管； 心肌病， 肥大性
［中图分类号］ R364.3＋1 ［文献标识码］ A
［文章编号］ 1000-4718(2000)10-0883-04
Role of cellular paracrine in the mechanism of cardiomyocyte
hypertrophy induced by stretch
FENG Bing
（Institute of radiation medicine, academy of military medicine， Beijing 100850, China；）
WANG De-wen
（Institute of radiation medicine, academy of military medicine， Beijing 100850, China；）
HE Zuo-yun
（Department of Cardiology, Xingiao Hospital, Third Military Medical University, Chongqing 400037, China）
［Abstract］ AIM： To investigate the role of cellular paracrine in the mechanism of cardiomyocyte hypertrophy induced by stretch. METHODS: ［3H］-leu incorporation into cultured cardiomyocytes was measured when stimulated by conditioned-media of myocardial fibroblast and microvascular endothelial cell after stretch. RESULTS： ［3H］-leu incorporation rate were both elevated significantly stimulated by conditioned-media of myocardial fibroblast and microvascular endothelial cell. And angiotensin II and endothelin of conditioned-media of myocardial fibroblast and microvascular endothelial cell were also elevated significantly. And ［3H］-leu incorporation rate were inhibited significantly when the specific angiotensin II and endothelin receptor antagonist losartan(1 μmol/L) and BQ123(1 μmol/L) were added. ［3H］-leu incorporation rate were inhibited over 80% when losartan(1 μmol/L) and BQ123(1 μmol/L) were added together. CONCLUSION: The activation of myocardial fibroblast and microvascular endothelial cell endocrine stimulated by stretch play a role through paracrine in the pathogenesis[1] [2] 下一页
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