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轻度修饰低密度脂蛋白对血管内皮细胞粘附功能影响及其机理初探 |
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刘春华 钱冠清 刘会齐 刘晓辉
［摘 要］ 目的：观察轻度修饰LDL (MM-LDL)对培养人脐静脉内皮细胞(HUVEC)与人类单核细胞系U937粘附功能及其表面粘附分子表达的影响。方法：利用计数法观察经MM-LDL作用的HUVEC与U937细胞的粘附率；用ELISA方法检测MM-LDL作用后HUVEC膜表面粘附分子血管细胞粘附分子-1(VCAM-1)、细胞间粘附分子-1(ICAM-1)及P选择素(P-selectin)的表达。结果：MM-LDL(75 mg/L)作用HUVEC 4 h后，其对U937细胞粘附率明显增加(P＜0.01)，HUVEC膜表面未见VCAM-1，ICAM-1，P-selectin表达上调，作为阳性对照重组肿瘤坏死因子α(rTNFα)5.0 μg/L可显著诱导以上3种粘附分子表达。延长MM-LDL与HUVEC作用时间至18 h可诱导产生P-selectin表达，对VCAM-1表达无影响。结论：MM-LDL诱导的HUVEC与U937粘附不是通过ICAM-1、VCAM-1介导的，P-selectin可能起一定的作用。
［主题词］ 内皮细胞；低密度脂蛋白类，LDL；粘连
［中图分类号］ R543.5； Q256 ［文献标识码］ A
［文章编号］ 1000-4718(2000)10-0887-04
Effect of mininally modified low density lipoprotein on the adhesive
function of vascular endothelial cell and its mechanism
LIU Chun-hua QIAN Guan-qing LIU Hui-qi LIU Xiao-hui
（nsitute of Hematology, Chinese Academy of Medical Sciences and
Peking Union Medical College, Tianjin 300020, China）
［Abstract］ AIM: To observe the effect of MM-LDL (minimally modified LDL) on the interaction between human umbilical vein endothelial cell (HUVEC) and U937 monocyte-like cell line and the exporession of vascular cell adhesion-1 (VCAM-1), intercellular adhesion molecule-1(ICAM-1), P-selectin.METHODS:The adhesive percentage between HUVEC treated with MM-LDL and U937 was determined by counting and the expression of VCAM-1, ICAM-1, P-selectin were examined by ELISA. RESULTS: Treatment of HUVEC with MM-LDL (75 mg/L) for 4 hours significantly increased adhesion of U937 to HUVEC (P＜0.01) and did not induce the surface expression of VCAM-1, ICAM-1, P-selectin . Recombination tumor necrosis factor-alpha (rTNFα) 5.0 μg/L， as a positive control, induced the expression of these adhesion molecules (P＜0.05). Prolonged (18 h) exposure to MM-LDL resulted in the expression of P-selectin, but not VCAM-1. CONCLUSION: the adhesion of monocytes to endothelial cells induced by MM-LDL is not mediated by VCAM-1, [1] [2] [3] 下一页
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