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P物质对气道平滑肌细胞增殖的调节作用 |
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吴昌归 毛宝龄 孙滨
摘 要 目的：观察P物质(SP)对犬气道平滑肌细胞(ASMC)增殖的调节作用。方法：以第四代ASMC为研究对象，分别以SP和［Sar9,Met(O2)11］-SP(NK-1受体兴奋剂)刺激，观察两者对细胞增殖的影响。又分别以GR 71251(NK-1受体拮抗剂)、新霉素［磷脂酶C(PLC)抑制剂］和尼群地平(钙离子通道阻断剂)为干预因素，观察它们对SP作用的影响。结果：SP和NK-1受体兴奋剂在10-7～10-5 mol/L范围内对犬ASMC呈剂量依赖性增生刺激作用。GR71251能明显抑制SP的促增殖作用，增殖抑制率为90.5%。新霉素(10-5 mol/L)和尼群地平(10-5 mol/L)对SP的促增殖作用抑制率分别为62.8%和40.3%，增加剂量抑制率不再增加。结论：SP可能通过NK-1受体，激活PLC，使细胞内三磷酸肌醇(IP3)和Ca2+浓度增加，促进ASMC增殖。
主题词 P物质； 肌，平滑；细胞； 狗
Regulatory effect of substance P on proliferation of airway smooth muscle cells
WU Chang-Gui, MAO Bao-Ling, SUN Bin
Department of Respiratory Disease, Xijing Hospital, the 4th Military Medical University, Xian(710032)
Abstract AIM:To investigate the effect of substance P(SP) on the proliferation of canine airway smooth muscle cells (ASMC). METHODS：The fourth generation of cultured cells were used in the study. Cells were cultured in DMEM. At 48 h post incubation, SP and ［Sar9, Met(O2)11］-SP, a specific agonist of NK-1 receptor, were added into the DMEM in varied concentrations, respectively. ［3H］-TdR incorporation rate was used as an index of cell proliferation. Additionally, we investigated whether the ASMC proliferative responses to SP were modulated by preincubation for 2 h with GR71251, a specific antagonist of NK-1 receptor, and neomycin, a phospholipase C(PLC) inhibitor, and nitrendipine, a blockader of Ca2+ channel, respectively. RESULTS：SP and NK-1 receptor-specific agonist induced dose-dependent proliferative response of the cells, the effect of SP was blockaded by GR71251, neomycin and nitrendipin(inhibition rate: 90.5%, 62.8% and 40.3%, respectively). The effects of neomycin and nitrendipine were not enhanced when their concentrations were increased. CONCLUSION：ASMC proliferative response to SP may be mediate by NK-1 receptor. SP binding NK-1 receptor can activate the PLC and cause increase of the concentration of IP3 and Ca2+ in ASMC.
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