|
甲基硝基亚硝基胍诱发的遗传不稳定细胞DNA聚合酶 mRNA表达水平研究 |
| |
冯朝晖 余应年 陈星若
摘 要 目的和方法：本实验室曾报道，低浓度0.2 μmol/L烷化剂甲基硝基亚硝基胍(N-methyl-N′-nitro-N-nitrosoguanidine, MNNG)可以诱导哺乳类细胞遗传不稳定状态。为明确其发生机制，本文利用定量RT-PCR方法，研究了该低浓度MNNG对人HeLa及猴肾vero细胞DNA聚合酶β(pol β) mRNA表达水平的影响。结果：经0.2 μmol/L MNNG 2.5 h处理后，HeLa细胞pol β mRNA水平在6～24 h内升高约1倍；而vero细胞pol β mRNA水平在12～30 h内亦升高1倍左右。结论：提示pol β mRNA水平改变可能参与MNNG诱发细胞遗传不稳定的发生。
主题词 诱变；DNA聚合酶类；基因表达；聚合酶链反应
mRNA expression of DNA polymerase β in MNNG induced genetically unstable cells
FENG Zhao-Hui, YU Ying-Nian, CHEN Xing-Ruo
Department of Pathophysiology and Laboratory of Molecular Biology, Zhejiang Medical University, Hangzhou (310031)
Abstract AIM and METHODS: It was reported from our laboratory that low concentration N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) could induce genetic instability in mammalian cells. To elucidate its mechanism, quantitative RT-PCR was used to study mRNA expression of DNA polymerase β in MNNG induced genetically unstable cells. RESULTS：It was demonstrated that mRNA level of polymerase β increased by nearly one-fold in HeLa cells from the 6th to 24th hour after being treated with 0.2 μmol/L MNNG for 2.5 h;and the same was demonstrated in vero cells from 12 th to 30 th hour after 0.2 μmol/L MNNG treatment. CONCLUSION：The alteration of polymerase β expression might be involved in the genesis of MNNG induced genetic instability in mammalian cells.
MeSH Mutagenesis; DNA polymerase; Gene expression; polymerase chain reaction
化学物质或离子辐射可以诱发哺乳类细胞遗传不稳定状态(genetic instability)，但其发生机制尚不明确［1］。本室证实低浓度0.2 μmol/L烷化剂甲基硝基亚硝基胍(N-methyl-N′-nitro-N-nitrosoguanidine, MNNG)处理细胞2.5 h，可诱发细胞遗传不稳定状态产生［2］；同时有DNA复制保真度明显下降［3］，及许多基因表达水平的改变［4］；转录抑制剂可阻断该状态产生［5］。提示其产生与有关基因表达水平改变有关。
许多研究提示DNA聚合酶β(pol β)可能与MNNG诱发细胞遗传不稳定有关。pol β具有较低的DNA复制保真度，及较强的跨损伤误性DNA修复等特性。MMS、 AAF、 H2O2、 TPA及大剂量MNNG(≥20 μmol/L)可诱导细胞pol β mRNA水平[1] [2] [3] 下一页
上一个医学论文： 大鼠细动脉平滑肌细胞内酸中毒对ATP敏感钾通道的影响
下一个医学论文： 表达反义hREV3 基因片段的真核细胞表达质粒的构建
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文