EB病毒BLLF1基因转基因小鼠淋巴瘤表型的观察

　    【摘要】  目的  观察EB病毒BLLF1基因转基因小鼠淋巴瘤的发病特征、组织病理学的特点及遗传稳定性。方法  用BLLF1基因制备了转基因小鼠，对其中2只MA表达阳性的发生淋巴瘤的鼠进行繁育并观察淋巴瘤的特征及遗传稳定性。结果  转基因鼠淋巴瘤的发病部位与人类发病部位相似，但出现顺序有不同，发病的性别差异与人类不同，经16代传代，淋巴瘤仍能稳定遗传。结论  该小鼠模型有可能成为探讨淋巴瘤发病机制的动物模型。

    【关键词】  EB病毒  淋巴瘤  转基因小鼠  遗传

    Phenotype study of lymphoma in EBV BLLF1 gene transgenic mice

       【Abstract】  Objective   To observe the pathogenic and histopathologic characteristics of lymphoma in EBV BLLF1 gene transgenic mice.Methods  We constructed transgenic mouse with EBV BLLF1 gene which codes for membrane antigen (MA) and found that two of the four lines that expressed  MA had the phenotype of lymphoma at the age of 4 month and 8 month old respectively. Analysis of pathology and immunologic phenotype of the lymphoma shows that it is T-cell lymphoma. Results  The  relationship between EBV BLLF1 gene and lymphoma for the first time，suggesting the expression of BLLF1 gene may lead to lymphoma. Here we provide the results of further study on the characteristics of lymphoma phenotype and the stability of lymphoma after breeding. Conclusion  The results show that after 16 passages， the phenotype of lymphoma is obvious and stable. 

    【Key words】  EB virus  lymphoma  transgenic mouse  heredity

    EB病毒（Epstein-Barr Virus ， EBV）在人群中的感染很普遍，流行病学调查以及临床病例研究发现EB病毒感染和人类若干种免疫系统的肿瘤密切相关，如地方性Burkitt’s淋巴瘤、Hodkin’s淋巴瘤及Non-Hodkin’s淋巴瘤［1］。

    近年来用转基因小鼠技术研究EB病毒和淋巴瘤的关系取得了一定成绩，英国Glasgow大学的Wilson JB（1996）用EB病毒核蛋白（nuclear antigen，NA）基因制备了转基因小鼠并发现其表达可引起淋巴瘤［2］。美国North Car

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