系统性红斑狼疮患者肿瘤坏死因子与HLA

彭学标　费虹明　徐文严

　　中国图书分类号　R593.24
　　摘　要　目的：探讨系统性红斑狼疮(SLE)的发病机理和遗传基础。方法：用聚合酶链反应结合顺序特异的寡核苷酸(PCR/SSO)探针杂交方法对71例汉族SLE患者和69例正常对照组HLA-DR亚区作DNA分型，同时用双抗体夹心ELISA法检测血清中TNFα水平。结果：发现汉族SLE患者中DR2频率显著高于正常对照组，而DR4频率则明显低于正常对照组，活动期SLE患者血清TNFα水平明显高于非活动期SLE患者和正常对照组，而非活动期SLE患者和正常对照组血清TNFα水平无明显差异。DR2阳性的SLE患者TNFα水平低，而DR4阳性的SLE患者TNFα水平则较高。结论：TNFα可能通过HLA-Ⅱ类基因或其单倍型在SLE的发病中起重要作用。
　　关键词　系统性红斑狼疮(SLE)　肿瘤坏死因子(TNF)　HLA-DR

Study on the association between HLA-DR and Tumor Necrosis Factor genes in the patients with Systemic Lupus Erythematosus

PENG Xue-Biao,FEI Hong-Ming,XU Wen-Yan.
Department of Dermatology,Nanfang Hospital,The First Military University,Guangzhou 510515

　　Abstract Objective：Study the pathogenesis and the genetic background of Systemic Lupus Erythematosus(SLE).Methods：To use polymerase chain reaction and sequence specific oligoncleotide(PCR/SSO) probe hybridization for typing HLA-DR subregion in 71 cases of patients with SLE of Han nationality and 69 cases of matched controls.At the same time,used ELISA of sandwich with two antibodies to examine Tumor Necrosis Factor-α(TNFα)in sera.Results:DR2 gene occured more frequent in patients than that of in controls,whereas DR4 gene occured less frequent in patients,compared with that of controls.TNFα level was much higher in the serum of SLE patients in the active stage than that of the patients and of that in the inactive stage and controls,but there was no difference between the latter.The level of TNFα was low in the patients with DR2 gene,but high in the with DR4 gene.Conclusion:Certain association of HLA-DR and TNF genes might be related in the pathogenesis of SLE.
　　Key words Systemic Lupus Erythematosus(SLE)　Tumor Necrosis Factor(TNF)　HLA-DR

　　肿瘤坏死因子(TNF)是体内具有多种生物活性的细胞因子，有抗肿瘤、抗病毒感染及免疫调节作用。在体外，TNFα可以诱导胰腺β细胞异常表达高密度MHC Ⅱ类分子，提示TNFα可能参与诱发自身免疫反应。系统性红斑狼疮(SLE)是原因不明的自身免疫性疾病，研究表明SLE的易感基因可能位于或靠

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