T细胞识别人多发性骨髓瘤细胞XG

江晓丰　张学光　李新燕　吴士良　

　　中国图书分类号　R392.12
　　摘　要　目的：探讨人多发性骨髓瘤细胞(MM)XG-7选择性激发扩增人外周血γδT细胞机制。方法：运用蛋白质分离纯化手段获取MM细胞XG-7膜蛋白组分，检查每一组分对γδT细胞的刺激效应，对其中的有效组分作Western blotting等鉴定分析。结果：证实了其中一种为热休克蛋白-70的膜蛋白能选择性扩增人外周血的γδT细胞。结论：表达于人多发性骨髓瘤细胞XG-7细胞膜上的热休克蛋白-70多肽是γδT细胞所识别的配体之一。
　　关键词　γδT细胞　热休克蛋白　肿瘤相关抗原　高效液相色谱层析仪

The recognition by human γδT cells of HSP70 peptide derived from XG-7 multiple myloma cell line
JIANG Xiao-Feng①,ZHANG Xue-Guang,LI Xin-Yan et al.Immunity Research Unit,Suzhou Medical College,Suzhou 215007.①Shanghai Chest Hospital,Shanghai 200030

　　Abstract Objective:To elucidate the mechanism of γδT cell’s response to human multiple myloma(MM) cell line XG-7,and to develop a novel expansion system of γδT cell by using PBMCs stimulated with HSP70 peptide derived from XG-7.Methods:The protein isolation and purification techniques were introduced.Results:A protein which specifically stimulated γδT cells were extracted and purificated.Furthermore,this effective protein was characterized as the HSP70 molecule by western blotting.Based on the findings,successfully developed a novel culture system that selectively expanded γδT cells by using PBMCs stimulated with HPS70-peptides.Conclusion:The HSP70-peptide on XG-7cells may be one of the ligand for γδT cells.
　　Key words γδT cell Heat shock protein(HSP) Tumor-associated antigen(TAA) HPLC

　　　自发现γδT细胞以来，人们一直在寻找γδT细胞所识别的抗原配体，一些人类γδT细胞克隆能以MHC限制方式识别表达于肿瘤细胞上自身Ig和白喉类毒素；而大多数γδT细胞则以非MHC限制性方式识别靶分子。早先的研究发现分枝杆菌及其抽提物能刺激人及小鼠的γδT细胞，一些与热休克蛋白60、65、70相关分子被证实是肿瘤细胞和分枝杆菌刺激γδT细胞的抗原配体［1，2］。
　　热休克蛋白及其相关分子能诱导有效的肿瘤免疫反应［3］。尽管其机制不是很明确，然而HSP及其相关分子能以某种未知机制为γδT细胞所识别，HSP致敏的γδT细胞能介导强烈的杀瘤效应，提示γδT细胞在HSP所诱导的肿瘤免疫反应中具有重要作用。
　　我们早先的研究显示γ&de

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