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亚急性重型肝炎患者TNF |
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张立煌 孙永良 石裕明 方海林 李敏伟 姚航平 刘克洲
摘 要 目的：分析IL-4对亚急性重型肝炎(亚重肝)患者外周血单个核细胞(PBMCs)TNF-α和IL-1的调控作用，评价IL-4在亚重肝治疗中的潜在价值。方法：应用细胞生物学、免疫组化和RT-PCR等方法测定PBMCs TNF-α、IL-1的表达。结果：亚重肝患者PBMCs TNF-α、IL-1表达水平均较正常人显著增高，虽然IL-4 mRNA及蛋白质的表达与正常人比较无显著性差异，但IL-4 mRNA/TNF-α mRNA和IL-4 mRNA/IL-1α mRNA的比值明显降低，IL-4以剂量依赖方式明显抑制LPS诱导的亚重肝患者PBMCs TNF-α、IL-1的表达；与正常人比较，IL-4对亚重肝患者PBMCs的作用效应降低。结论：IL-4对亚重肝PBMCs TNF-α、IL-1的表达均具有明显抑制作用，在亚重肝的治疗上具有潜在的应用价值。
关键词 亚急性重型肝炎 白细胞介素 4 肿瘤坏死因子 α 白细胞介素 1 外周血单个核细胞
中国图书分类号 R512.62 R392.11
Study on effect of IL-4 on expression of TNF-α and IL-1 from patients with subfulminant hepatitis
ZHANG Li -Huang, SUN Yong -Liang, SHI Yu -Ming et al. Institute of Infectious Diseases, Zhejiang Medical University, Hangzhou 310003
Abstract Objective: To study the effect of IL-4 on expression of TNF-α and IL-1 by peripheral blood mononuclear cells (PBMCs) from patients with subfulminant hepatitis (SFH) and estimate the potential clinical value of IL-4 in treatment of SFH. Methods:The expression of TNF-α and IL-1 by PBMCs was detected by biological activity assay,immunohistochemical assay and semiquantitative reverse transcriptional polymerase chain reaction (RT-PCR).Results:The expression of TNF-α and IL-1 by PBMCs from patients with SFH were greatly enhanced as compared with that of normal controls. Although there was no difference in expression of IL-4 mRNA/IL-1α mRNA and IL-4 mRNA/TNF-α mRNA in SFH were lower than in normal controls.IL-4 in dose-dependent manner down regulated the LPS-stimulated produced of both TNF-α and IL-1 by PBMCs from patients with SFH. It was also found that the response of PBMCs to IL-4 decreases in SFH as compared with the normal controls.Conclusion:IL-4 seems to be a potent down-regulator of TNF-α and IL-1 and may have a potential role in treatment of SFH.
Keywords Subfulmi[1] [2] [3] 下一页
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