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血管紧张素 对心肌梗塞大鼠成纤维细胞的核酸 胶原合成影响的研究 |
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申景平 雷立权 高广道 李瑞峰 李 炯 张庆殷
摘 要 目的和方法：本实验利用酶法分离、培养成年大鼠心肌成纤维细胞(Fbs),观察了血管紧张素Ⅱ(Ang Ⅱ)在不同处理因素下对Fbs的3H-TdR、14C-UR、3H-Pro掺入率的影响。结果：在相同浓度(10-7mol/L)Ang Ⅱ作用下，MI组Fbs对上述3种标记底物的掺入率均显著高于假手术(SO)组(P＜0.05)。Ang Ⅱ的上述作用，可被特异性Ang Ⅱ拮抗剂［1.8］Ang Ⅱ和血管紧张素抗肽(Ang-AP)完全阻断，却不能被Losartan完全阻断。结论：Ang Ⅱ可直接作用于心肌Fbs,促进Fbs的DNA、RNA和胶原蛋白的合成。MI组大鼠Fbs对Ang Ⅱ反应性显著高于SO组，介导Ang Ⅱ对Fbs的作用除AT1外，可能还有其他机制参与。
主题词 心肌梗塞；血管紧张素Ⅱ；成纤维细胞
Effect of angiotensin Ⅱ on the syntheses of DNA, RNA and collagen
protein in isolated fibroblasts from myocardium-infarcted rats
SHEN Jing-Ping, LEI Li-Quan, GAO Guang-Dao, LI Rui-Feng, LI Jiong, ZHANG Qing-Yin
Department of Pathophysiology, Shandong Medical University, Jinan (250012)
Abstract AIM and METHOD: Isotope-labelled substrate incorporation method was used to investigate the effect of angiotensin Ⅱ(Ang Ⅱ) on the syntheses of DNA, RNA and collagen protein of isolated fibroblasts(Fbs) from myocardial infarction (MI) and sham operated (SO) rats in different conditions. RESULTS: The incorporating rates of 3H-thymidine, 14C-uridine and 3H-proline of Fbs from MI group were significantly higher than that of SO group, respectively. These effects of Ang Ⅱ on Fbs were abolished completely either by angiotensin antagonists, 1.8Ang Ⅱ, or by angiotensin antipeptide. On the other hand,angiotensin receptor antagonist, losartan, can not thoroughly eliminate the above-mentioned effects of Ang Ⅱ. CONCLUSIONS: (1) Ang Ⅱ promoted the syntheses of DNA, RNA and collagen protein in Fbs; (2) The responsiveness of Fbs to Ang Ⅱ from MI rats was higher than that of normal rats; (3)AT1 receptor is not the only substype which regulates the action of Ang Ⅱ to Fbs metabolism.
MeSH Myocardial infarction; Angiotensin Ⅱ; Fibroblasts
近年来的研究表明，决定心肌梗塞(myocardial infarction, MI)后心脏功能和病人预后的主要因素是左室改建(left ventricular remodeling, LVR),即MI后发生的，以左室为主的空间构型、组织结构、机能与代谢、基因表达与调控等一系列改变的病理过程。其突出特点为非梗塞区心肌肥大和左室腔扩大［1］。心脏局部的肾素[1] [2] 下一页
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