|
杂色曲霉素诱导体外培养人外周血淋巴细胞凋亡的研究 |
| |
曹文军 孙旭明 张祥宏 严 霞 王俊灵 王凤荣
摘 要 目的：研究我国肿瘤高发区饮食污染霉菌毒素-杂色曲霉素(ST)对人免疫机能的可能影响。方法：流式细胞术(flow cytometry, FCM)、形态学观察及DNA电泳。结果：ST可诱导体外培养的人外周血淋巴细胞发生凋亡，并且在一定时间(2～48 h)及剂量(0.125～2.0 mg/L)范围内呈正相关关系。光镜及透射电镜观察均发现了凋亡细胞典型的形态学变化，如核染色质凝集边集，凋亡小体形成等；DNA琼脂糖凝胶电泳也出现特征性的DNA Ladder条带。结论：ST可诱导人外周血淋巴细胞发生凋亡，从而可能在一定程度上影响机体的免疫机能。
主题词 流式细胞术；人类；淋巴细胞
Effects of sterigmatocystin on apoptosis of human
peripheral blood lymphocytes in vitro
CAO Wen-Jun, SUN Xu-Ming, ZHANG Xiang-Hong, YAN Xia,WANG Jun-Ling, WANG Feng-Rong
Department of Pathology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang (050017)
Abstract AIM:To explore the possible impacts of sterigmatocystin (ST) on human immunological system. METHODS:Flow cytometric (FCM) analysis, morphological observation and DNA electrophoresis. RESULTS:FCM analysis revealed that the apoptotic rates of all the lymphocytes in ST treated groups were significantly higher than those in their corresponding control groups. Significant dose-effect and time-effect correations could be found between apoptotic rates and ST concentrations in the range from 0.125 mg/L to 2 mg/L as well as ST treating time from 2 h to 48 h in the same concentrations (2 mg/L). Further works showed that under light and electron microscope, chromatin condensation and margination alongside nuclear membrane, nuclear membrane disintegration, nuclear fragmentation as well as apoptotic bodies were found in ST treated cells in semi-ultrathin and ultrathin sections. While in agarosegel electrophoresis, a characteristic “ladder” pattern caused by products of DNA degradation was also found in ST treated cells. CONCLUSION:ST could induce apoptosis in human peripheral blood lymphocytes in vitro. Thus it might have some negetive impacts on human immunity.
MeSH Flow cytometry; Human; Lymphocytes
杂色曲霉素(sterigmatocystin, ST)是我国恶性肿瘤高发区居民饮食中污染率较高的一种致癌性霉菌毒素［1～4］。为探讨ST对恶性[1] [2] 下一页
上一个医学论文： 边缘系创伤反应性髓鞘碱性蛋白表达与超微结构观察
下一个医学论文： 低浓度H2O2对肺微血管内皮细胞的迟发效应 凋亡
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文