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RCS大鼠视网膜感光细胞的凋亡 |
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图2 RCS大鼠40 d视网膜，PRC外节层(B)增宽，排列紊乱，PRC内节层(C)变窄，出现空泡，外核层(D)明显变窄，散在细胞核碎块，视网膜色素上皮细胞(A)排列轻度紊乱，外核层和视杆视锥层可见类巨噬细胞的侵入(箭头)。HE ×200
图3 RCS大鼠100 d视网膜，RPE层(A)明显增殖，排列紊乱，PRC外节、内节层及外核层几乎消失，内核层(E)及神经节细胞层(F)保持基本正常结构。HE ×200
Fig.1 The retina of SD rat of 35 days old. The structure of retinal pigment epithelium (A), outer segment of PRC (B), inner segment of PRC (C), outer nuclear layer (D), inner nuclear layer (E) and ganglion cell layer (F) were all normal. HE ×200
Fig.2 The retina of RCS rat of 40 days old. The outer segment zone of PRC(B) became elongated and irregularly aligned. The inner segment zone of PRC(C) was shortened with vacuolation. The outer nuclear layer (D) was obviously thicken and had many nuclei debris. The retinal pigment epithelial cells (A) were slightly misaligned. A few macrophage-like cells invaded into the outer nuclear layer and rod or cone layer (arrow). HE ×200
Fig.3 The retina of RCS rat of 100 days old. The retinal pigment epithelial cells (A) proliferated and misaligned, the inner and outer segment zone of PRC, and the outer nuclear layer almost disappeared, the inner nuclear layer (E) and the ganalion cell layer (F) kept normal structure. HE ×200
2.TUNEL检测结果
各鼠龄SD大鼠视网膜均为阴性反应。RCS大鼠视网膜，从出生后25 d起，外核层的少许PRC胞核呈TUNEL反应阳性；30 d时，阳性细胞数增加；35 d时达高峰，部分胞核染色呈环状或呈均匀一致状(图4)；40 d时，阳性细胞数减少，染色多呈均一状。60 d时偶见阳性反应的细胞核，以后几乎检测不到。
Fig.4 The retina of RCS rat of 35 days old. Many nuclei of photorecptors were labeled (arrow) by TUNEL methods. TUNEL staining ×250
图4 RCS大鼠35 d视网膜，外核层有大量呈TUNEL染色阳性的细胞核(箭头)。TUNEL染色 ×250
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