|
谷氨酰胺对缺氧复氧损伤人小肠上皮细胞谷胱甘肽的影响 |
| |
戴定威 吴圣楣 戚秋芬 李 敏 陈惠金 蔡 威
摘 要 目的：探讨谷氨酰胺(GLN)对缺氧复氧损伤人小肠上皮细胞(IEC)内还原型谷胱甘肽(GSH)和丙二醛(MDA)的影响。方法：采用原代培养人IEC作为实验模型，用荧光法测定体外培养IEC内GSH和MDA含量。结果：正常对照组GSH含量最高，在对照组加入GLN并不影响GSH含量(P＞0.05)。缺氧60 min或90 min复氧30 min损伤IEC细胞内GSH含量均显著低于正常对照组(P＜0.05)，预先应用GLN可显著阻止缺氧复氧损伤IEC细胞GSH含量下降(P＜0.05)。相反，正常对照组MDA含量最低，缺氧60 min复氧30 min IEC细胞内MDA水平显著高于正常对照组(P＜0.01)，预先应用GLN可显著降低缺氧复氧损伤IEC细胞内MDA水平(P＜0.05)。结论：补充GLN可维持缺氧复氧损伤IEC细胞GSH水平，减轻细胞膜脂质过氧化。
主题词 肠，小； 上皮； 细胞； 低氧； 谷氨酰胺； 丙二醛
Effects of glutamine on intracellular reduced glutathione in cultured human intestinal epithelial cells with anoxia/reoxygenation
DAI Ding-Wei, WU Sheng-Mei, QI Qiu-Fen, LI Min, CHEN Hui-Jin, CAI Wei
Shanghai Institute for Pediatric Research, Xinhua Hospital, Shanghai Second Medical University, Shanghai (200092)
Abstract AIM：To study the effects of glutamine on intracellular reduced glutathione (GSH) and malondialdehyde (MDA) in cultured human intestinal epithelial cells with anoxia/reoxygenation (A/R) in vitro.METHODS: Human intestinal epithelial cells in primary culture was used as a model and intracellular GSH and MDA were determined by using fluorometric method.RESULTS: Normal control group had the highest GSH level and there was no difference between NS vs 2 mmol/L glutamine group (P＞0.05)。 With 60 min or 90 min of anoxia followed 30 min of reoxygenation, GSH levels were significantly lowered in NS groups compared to those in glutamine groups (P＜0.05). Normal control group had the lowest MDA level. With 60 min of anoxia followed 30 min of reoxygenation, MDA level was significantly higher in A/R group compared to that in glutamine group (P＜0.05). CONCLUSIONS: Glutamine supplementation might partially preserve GSH levels and lessen lipid peroxidation of cell membrane in human IEC during A/R in vitro.
MeSH Intestine, small; Epithelium; Cells; Anoxia; Glutamine; Malondialdehyde
缺血所致小肠损伤的主要原因之一是再灌注时生成氧自由基(oxygen free radical, OFR)[1] [2] [3] 下一页
上一个医学论文： 免疫调节剂对大鼠巨噬细胞脂质过氧化物和酸性磷酸酶的影响
下一个医学论文： 肺表面活性物质对大鼠肺损伤后氧合功能的影响
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文