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牛磺酸对缺血大鼠心肌线粒体Ca2 |
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江西医学院生化教研室(南昌 330006) 万福生 赵小曼 雷 厉 龚文华
摘 要 目的和方法：用Wistar大鼠皮下注射异丙肾上腺素(ISP，5 mg/kg)诱导心肌缺血模型。观测心肌线粒体(Mit)中丙二醛(MDA)含量、Ca2＋-Mg2＋-ATP酶和Ca2＋-ATP酶活性及牛磺酸(Tau)的影响。结果：缺血组大鼠心肌Mit中MDA升高87.83%、Ca2＋-Mg2＋-ATP酶和Ca2＋-ATP酶活性分别降低37.56%和50.20%(P＜0.01)。Ca2＋-Mg2＋-ATP酶活性与MDA含量也呈显著负相关(r=-0.87,P＜0.01)。Ca2＋-ATP酶活性与MDA含量也呈显著负相关(r=-0.79，P＜0.01)。在注射异丙肾上腺素(ISP)前30 min腹腔注射Tau(200 mg/kg)则Mit中MDA含量、Ca2＋-Mg2＋-ATP酶和Ca2＋-ATP酶活性均未见显著异常改变。结论：Tau可能通过抑制MDA的生成实现其保护Ca2＋-ATP酶和Ca2＋-Mg2＋-ATP酶活性的作用。
主题词 牛磺酸；腺苷三磷酸酶；丙二醛；线粒体；心肌
Effect of taurine on Ca2＋-Mg2＋-ATPase activity and MDA content of
myocardial mitochondria in ischemic rats
WAN Fu-Sheng, ZHAO Xiao-Man, LEI Li, GONG When-Hua
Department of Biochemistry, Jiangxi Medical College, Nanchang (330006)
Abstract AIM and METHOD: Myocardial ischemia model was induced by subcutaneous injection of isoproterenol (ISP. 5 mg/kg) into Wistar rats. MDA (malondialdehyde) content and activities of Ca2＋-Mg2＋-ATPase and Ca2＋-ATPase were observed. RESULTS: Compared with the control, MDA content was increased by 87.83%; Ca2＋-Mg2＋-ATPase and Ca2＋-ATPase activities were decreased by 37.56% and 50.20% respectively. A definite negative correlation (r=-0.87, P＜0.01) between MDA content and Ca2＋-Mg2＋-ATPase activity as well as (r=-0.79, P＜0.01) between MDA content and Ca2＋-ATPase activity were found. Taurine was injected intraperitoneraly 30 min before injection of ISP, the MDA content and Ca2＋-Mg2＋-ATPase and Ca2＋-ATPase activities in the myocardial mitochondria had not much change. CONCLUSION: Protective effect of taurine on Ca2＋-Mg2＋-ATPase and Ca2＋-ATPase activities were completed by reducing MDA formation of mitochondria.
MeSH Taurine; Adenosine triphosphatase; Malondialdehyde; Mitochondria; Myocardium
牛磺酸(taurine,Tau)属含硫的β-氨基酸，是哺乳动物心脏含量最丰富的自由氨基酸。近年发现［1，2］，在缺血(氧)等情况下，心肌细胞Tau含量下降，且与心肌损伤程度密切相关。缺乏Tau的动物心脏对缺血及再灌注所致心肌损伤的敏感性也明显增加。在各种心肌损伤中，线粒体(mitochondria, Mit)往往首当其害。我们曾在异丙[1] [2] 下一页
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