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不同病期支气管哮喘血清及PBMC条件培养液IL |
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刘 凤 冯学斌 黄 薇 房崇云 于树莉 马永红 傅振荣
摘 要 目的：探讨IL-8、TNF-α在儿童支气管哮喘发病中的作用。方法：采用酶联免疫吸附试验(ELISA)检测35例发作期、18例稳定期哮喘患儿血清及外周血单个核细胞(PBMC)条件培养液中IL-8、TNF-α水平。结果：哮喘发作期血清IL-8及TNF-α明显增高，随病情缓解逐渐降低，达到或接近对照组水平。与对照组比较，哮喘发作期、稳定期PBMC条件培养液IL-8、TNF-α水平显著增高；无论发作期抑或稳定期哮喘患儿PBMC经PHA刺激所产生的TNF-α、均显著高于未加PHA刺激所产生的水平，而PBMC产生的IL-8则无类似变化。结论：儿童哮喘存在IL-8、TNF-α合成或释放失调，二者可能参与哮喘气道慢性炎症的发生发展过程。
主题词 哮喘；白细胞介素8；肿瘤坏死因子；儿童
Change of IL-8，TNF-α in serum and supernatant of peripheral blood mononuclearcytes from children with bronchial asthma in different periods
LIU-Feng,FENG Xue－Bin,HUANG Wei，FANG Chong-Yun, YU Shu-Li, MA Yong-Hong, FU Zhen-Rong
Department of Pathophysiology, Binzhou Medical College, Shandong Binzhou (256603)
Abstract AIM：To explore the effect of IL-8 and TNF-α in children with asthma.METHODS：Levels of IL－8、TNF-α in serum and supernatant of peripheral blood mononuclearcytes (PBMC) were measured using enzyme linked immunosorbent asssy (ELISA) in children with bronchial asthma during attack (35 cases) and stable (18 cases) conditions. RESULTS：Levels of IL-8 and TNF-α were significant increased during asthma attack and were decreased to or near the levels of control in companying with asthma remission. Compared with control, the levels of IL-8，TNF-α in supernatant of PBMC were raised obviously during asthma attack and stable. Wheather asthma attack or stable, the level of TNF-α from PBMC stimulated by PHA (phytohemagglutinin)were higher than that which was not stimulated by PHA, but the IL-8 from PBMC was not changed like TNF-α.CONCLUSION：There existed irregularity in production or releasing of IL-8, TNF-α in asthma.IL-8 and TNF－α may take part in the progress of chronic airway inflammation in asthma.
MeSH Asthma; Interleukin-8; Tumor necrosis factor; Child
支气管哮喘(bronchial asthma)是小儿常见的慢性肺部疾病。也是造成青少年生活质量下降的主要疾病影响因素。[1] [2] 下一页
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