|
大鼠脑干向面口部运动核的P物质能和脑啡肽能投射 |
| |
李云庆 王智明 施际武
【摘要】 目的 观察大鼠脑干面口部运动核(三叉神经运动核、面神经核和舌下神经核)内P物质(SP)和亮氨酸脑啡肽(L-ENK)样阳性终末的中枢起源。 方法 荧光金(FG)逆行追踪与SP样和L-ENK样免疫荧光组织化学染色相结合的双重标记技术。 结果 将FG分别注入三叉神经运动核、面神经核或舌下神经核后，FG逆标神经元主要位于臂旁核簇、三叉上核、三叉神经感觉主核、脑桥和延髓网状结构、巨细胞网状核α部和延髓中缝核簇。上述结构内的部分FG逆标神经元呈SP样或L-ENK样阳性，这些双标神经元主要位于臂旁外侧核、延髓中缝核簇和巨细胞网状核α部。 结论 含SP和L-ENK并向脑干面口部运动核投射的运动前神经元可能在面口部复杂而精细的运动调控中发挥重要作用。
【关键词】 脑干面口部运动核 运动前神经元 P物质 脑啡肽 大鼠
SUBSTANCE P-ERGIC AND ENKEPHALINERGIC PROJECTIONS
FROM THE BRAINSTEM TO THE BRAINSTEM OROFACIAL
MOTOR NUCLEI IN THE RAT
Li Yunqing△,Wang Zhiming,Shi Jiwu
(Department of Anatomy and K.K.Leung Brain Research Centre,
The Fourth Military Medical University,Xi an)
【Abstract】 Objective To study the central origins of substance P-ergic and enkephalinergic projections from the brainstem to the brainstem orofacial motor nuclei in the rat.Methods The double-labeling method by combination of Fluro-Gold(FG) retrograde tracing with immunohistochemical staining for substance P(SP) and leucine-enkephalin (L-ENK) was used in the present study.Results After injection of FG into the trigeminal motor nucleus,facial nucleus,or hypoglossal nucleus,respectively,FG retrogradely labeled neurons were found mainly in the parabrachial nuclei,supratrigeminal nucleus,trigeminal principal sensory nucleus,pontine and medullary reticular formation,medullary raphe nuclei and gigantocellular reticular nucleus pars α.Among FG retrogradely labeled neurons in those structures as mentioned above,some of the FG labeled neurons also showed SP- or- L-ENK-like immunoreactivities.SP/FG and L-ENK/FG double-labeled neurons were mainly encountered in the lateral parabrachial nucleus,medullary raphe nuclei and gigantocellular reticular nucleus pars α,which are important structures of the premotor neuron pool.Conclusion SPergic and L-ENKergic projections originated mainly from brainstem premotor neuron[1] [2] [3] 下一页
上一个医学论文： 烫伤大鼠室旁核和触液神经元中胆囊收缩素的变化
下一个医学论文： 大鼠大脑运动皮质与脑干面口部运动前神经元的突触联系
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文