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大鼠心脏腺苷预处理琥珀酸脱氢酶电镜细胞化学的变化 |
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张友云 董峰 郑汉巧 冯晓祥 卢彦珍 胡成俊
【摘要】 目的 以外源性腺苷前质——硫酸腺嘌呤注入大鼠，通过琥珀酸脱氢酶(SDH)电镜细胞化学的变化，探讨预处理效应。 方法 实验采用体重为200～300g雄性SD大鼠24只，分4组，每组6只，即正常对照组(Ⅰ组)；缺血再灌组(Ⅱ组)；缺血预处理组(Ⅲ组，按经典的Murry法)；硫酸腺嘌呤预处理组(Ⅳ组)。按Nitro-BT法显色制作SDH光镜标本，按小川的亚铁氰化铜法制作电镜标本。光镜观察SDH活性参照Niles法分为4级。 结果 Ⅰ组SDH活性和心肌结构相对正常；Ⅱ组SDH活性降低明显，线粒体损伤严重；Ⅳ组近于Ⅰ组；Ⅲ组界于Ⅰ、Ⅳ组之间。 结论 外源性硫酸腺嘌呤注入大鼠具有与经典缺血预处理相类似的抗心肌缺血再灌损伤的效应。
【关键词】 硫酸腺嘌呤 缺血预处理 琥珀酸脱氢酶 心脏 大鼠
THE STUDY OF SDH ELECTRON MICROSCOPIC CYTOCHEMISTRY
ON PRECONDITIONING OF RAT HEART CAUSED BY ADNOSINE
Zhang Youyun△，Dong Feng**，Zheng Hanqiao**，Feng Xiaoxiang，
Lu Yanzheng**，Hu Chenjun
(Department of Anatomy,**Department of Physiology,Hubei Medical University,Wuhan)
【Abstract】 Objective This paper aimed at exploring ischemic preconditioning(IPC) effect of exogenous preadenosine(adenine sulphate)by means electron microscopy of succinic dehydrogenase(SDH) to provide some experimental data for non-injury IPC method.Methods 24 male SD rats,weighting 200-300g,were divided into 4 groups,each of which consists of 6 rats.They are normal control group(Ⅰ group);ischemic/reperfusion group(Ⅱgroup);ischemic preconditioning group(Ⅲ group,Murry method)and adenine sulphate preconditioning group(Ⅳ group).After finishing the operation the myocardium of left ventricle was frozen sectioned and demonstrated SDH activity with Nitro-BT method.The electron microscopic samples for SDH activity were prepared according to ferrocyanide copper of Ogawa.The activity of SDH was divided into 4 scales based on Niles classification.Results The result indicated that as compared with group Ⅰ the injury on mitochondria and the decrease on SDH activity were the most serious in group Ⅱ,but is diminished greatly in group Ⅳ.The changes of group Ⅲ is between the two groups above.Conclusion The data suggested that injection of exogenous adenine sulphate into rats showed the anti-injury in myocardium caused by ischemic/[1] [2] 下一页
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