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非创伤缺血预处理对大鼠缺血再灌注心肌的作用 |
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卢彦珍* 董传仁 张友云** 郑汉巧 涂淑珍 欧阳静萍
摘 要 目的：确定非创伤性缺血预处理对大鼠缺血/再灌注(I/R)心肌损伤是否具有对抗作用，以扩展缺血预处理的实际应用。方法：采用非创伤性下肢缺血预处理及经典缺血预处理的动物模型，比较两种处理方法对I/R心肌损伤的效应。实验动物分4组：正常对照组(NC，n=8),开胸旷置50 min；缺血/再灌注组(I/R，n=12),结扎冠脉30 min，再灌注20、180 min；经典缺血预处理组(C-IPC，n=12)，按经典Murry法复制；非创伤性下肢缺血预处理组(N-WIPC，n=12)，捆绑双下肢5 min，松开5 min，反复4次后，阻断冠脉30 min，再灌20、180 min。以左室功能，心肌梗塞范围，血清肌酸激酶(CK)及心肌组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性为观察指标。结果：与I/R组相比，N-WIPC组与C-IPC组均显著缩小I/R后的心肌梗塞范围(P＜0.01)；明显恢复I/R后的左室功能(P＜0.05，0.01)；减轻自由基对心肌的损害：血清CK，心肌MDA含量显著降低(P＜0.01)。N-WIPC组还使心肌SOD活性增高(P＜0.05)。结论：非创伤性下肢缺血预处理与经典缺血预处理可诱发同等强度的心肌预处理效应。
主题词 大鼠；心肌;再灌注损伤
Effects of non-wounded ischemic preconditioning on myocardium ischemic reperfusion injury in rats
LU Yan-Zhen,DONG Chuan-Ren,ZHANG You-Yun,
ZHENG Han-QIAO, TU Shu-Zhen, OUYANG Jing-Ping
Department of Pathophysiology,Hubei Medical University, Wuhan(430071)
Department of Pathophysiology,Changzhi Medical College, Changzhi(046000)
Abstract AIM：To determine whether the non-wounded legs repeated-brief ischemic preconditioning has protective effects to extend the further application of IPC.METHODS：Two animal models which were the non-wounded legs repeated-brief ischemic preconditioning and the classical ischemic preconditioning,were produced to be compared with their protective effects on myocardial ischemic-reperfusion injury.Rats were divided into four groups:normal control group (NC,n=8),ischemic-reperfusion group (I/R,n=12), calssical ischemic preconditioning(C-IPC,n=12), and non-wounded legs ischemic preconditioning group(N-WIPC,n=12).Changes in left ventricular functions,myocardial infarct size,creatine kinase(CK) in serum and MDA，SOD of myocardium were investigated. RESULTS：Compared with I/R group， non-wounded legs ischemic preconditioning and classical ischemic preconditioning could: (1)Reduce the myocardial infarct size(P＜0.01);(2)Improve the left ventricular function;(3)Decrease the content of MDA of m[1] [2] 下一页
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