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病毒激活巨噬细胞产生的一氧化氮与细胞毒的关系 |
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白莲花 于春 刘倩 李娟 鲍秋莉 陈乃玲
摘 要 目的：确定新城鸡瘟病毒Losota系(NDV-L)能否诱导小鼠腹腔巨噬细胞(PEMφ)产生一氧化氮(NO)以及该NO与小鼠PEMφ介导的细胞毒作用是否有依赖关系。方法：采用Griess、FACS分析和噻唑蓝(MTT)方法。结果：NDV-L与PEMφ作用2 h后就可以稳定地吸附在PEMφ表面，吸附率达80%左右；收集NDV-L与PEMφ作用24 h上清，发现有NO的释放，释放量与阳性对照组(BCG-LPS-PEMφ上清)的释放量接近；采用MTT法测定NDV-L-PEMφ对H22肝癌细胞、P815肥大细胞瘤、Raji细胞和K562白血病细胞的细胞毒活性(效／靶比为20∶1)，发现NDV-L-PEMφ对这些靶细胞具有明显的杀伤活性，且该活性与NDV-L-PEMφ诱导NO之间有一定的依赖性。结论：新城鸡瘟病毒Losota(NDV-L)能诱导小鼠巨噬细胞产生和释放一氧化氮，且该一氧化氮与巨噬细胞的细胞毒活性具有依赖关系。
关键词 一氧化氮 新城鸡瘟病毒 细胞毒活性
The study of relationship between Nitric Oxide(NO) and the cytotoxic activies of peritoneal elicited macrophage(PEM) induced by Newcastle Disease Virus losota strain(NDV-L)
BAI Lian-Hua, YU Chun, LIU Qian et al. Institute of Liver Disease, Beijing Army General Hospital，Beijing 100700
Abstract Objective:Determine if NDV-L can induce mouse PEMφ releasing Nitric Oxide (NO) and the relationship between the NO and the cytotoxic activity of PEMφ. Methods:Used Griess FACS and MTT analysis. Results:NDV-L could absorb steadily on the surface of PEMφ after infecting PEMφ for 2 h and absorption ratio was about 80 percent. When detecting the supernatant liquid which NDV-L infected PEMφ for 24 h found that the quantities of NO was almost equal to the positive control group (BCG-LPS-PEMφ); While using MTT assay also found that the cytotoxic activities of NDV-L-PEMφ against H22, P815, K562 and Raji targets was much higher than the ones of control group and there were some dependent relationship between NO and the cytotoxic activities of PEMφ. Conclusion:Newcastle Disease Virus can induce macrophages producing Nitric Oxide (NO) and there was relationship between the NO and the cytotoxic activities of PEMφ.
Key words Nitric Oxide Newcastle Disease Virus Cytotoxicity
巨噬细胞是一种非特异性的免疫细胞，激活以后可具有较强的杀伤活性。近年来将其作为抗原提呈细胞(Antigen presenting cells, APC)和过继转输的免疫活性细胞[1] [2] 下一页
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