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人胚胎胰岛分泌白细胞介素6的动力学研究 |
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孙 氵内 田志刚 张建华 杨贵贞△
摘 要 目的：阐述IL－6与胰岛功能或胰岛病变的可能关系。方法：采用常规消化方法分离人胚胎胰岛并进行原代培养，培养上清进行IL－6、胰岛素、胰高血糖素活性测定和IL－6 McAb中和实验，同时对胚胎胰岛进行体外葡萄糖刺激实现。结果：原代培养的胚胎胰岛在40 h内IL－6分泌呈上升趋势(37～153 mU/胰岛)，每24 h重新换取新培养液作为一个分泌周期，IL－6分泌以第1周期最高(106 mU/胰岛)，体外连续培养IL－6的分泌以1 d最高(145 mU/胰岛)，用IL－6 McAb可以阻断胰岛培养上清中IL－6的活性；胰岛素、胰高血糖素的分泌周期与IL－6不同；葡萄糖刺激明显促进IL－6的分泌，其分泌趋势与胰岛素分泌一致。结论：原代培养的人胚胎胰岛在无任何刺激情况下可自发分泌IL－6，而且IL－6的分泌有自己的分泌环路。
主题词 人类；胚胎；胰岛；白细胞介素－6
IL-6 secretion kinetics of human fetal islets
SUN Rui, TIAN Zhi-Gang, ZHANG Jian-Hua, YANG Gui-Zhen
Shandong Cancer Biotherapy Center, Shandong Academy of Medical Sciences, Jinan 250062)
Abstract AIM:To study the relationship between IL-6 and function of pancreatic islets, IL-6 secretion kinetics of hmuan fetal pancreatic islets was investigated.METHODS:Islets from fetal pancreas were separated by collagenase type V (0.5 g/L) and cultured in vitro. The supernatants of primary culture of hmuan fetal islets for different days were assayed for IL-6, insulin and glucagon. The islets were stimulated by glucose and the supernatants was assayed too.RESULTS:IL-6 content of islet supernatant increased and peaked at 40 hour after stimulation (from 37 mU/islet to 153 mU/islet). Islets were replenished every 24 hours and secretion in first 24 h was highest (106 mU/islet). Cumulative Il-6 content in culture was highest on day 1 (145 mU/islet). IL-6 secretion was promoted dose-dependently after adding glucose to islets culture and insulin secretion was paralleled to IL-6 production.CONCLUSION:Islets may secret IL-6 spontaneously, and the glucose stimulation may regulate the IL-6 secretion, which is related to insulin production.
MeSH Human;Embryo; Islands of Langerhans; Interlukin-6
IL－6对免疫系统、神经系统、肝脏代谢、造血系统均具有正向调节效应。近年来开始有人通过动物实验探讨IL－6与胰岛功能及胰岛病变的关系，提示IL－6可能与胰岛素分泌以及与糖尿病发病学有关［1～3］。1993年我们初步观察到正常人胚胎胰岛细胞可自发分泌IL－6［4］，本文拟介绍人胚胎胰岛分泌IL－6的动力学特征，为进一步认识IL－6的功能提供依据。
材料和方法
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