|
柯萨基B3病毒感染增强单个核细胞与心脏微血管内皮细胞的粘附 |
| |
沈 茜 徐玉莲 凌 伟 吴 泓
中国图书分类号 R392.12
摘 要 目的：研究柯萨基B3病毒(Cox B3)感染对内皮细胞表面ICAM-1表达的诱导作用，及对内皮细胞与单个核细胞粘附的影响，探讨病毒性心肌炎心肌细胞免疫损伤的机理。方法：采用流式细胞仪分析内皮细胞ICAM-1表达；粘附试验和单克隆抗体抑制试验观察单个核细胞与内皮细胞的粘附。结果：100和400 TCID50／ml Cox B3感染内皮细胞24和48 h，均可显著增加单个核细胞与内皮细胞的粘附(P＜0.01)，分别为对照组的1.8和2.5倍。经流式细胞仪分析证实，Cox B3感染可以诱导内皮细胞ICAM-1表达增加，阳性细胞数增多。用抗ICAM-1和抗LFA-1单克隆抗体均可部分阻断单个核细胞与Cox B3感染内皮细胞的粘附，抗ICAM-1单抗的抑制作用较抗LFA-1单抗更为明显。结论：Cox B3可以通过感染内皮细胞上调膜ICAM-1的表达，促进单个核细胞与内皮细胞的粘附。
关键词 柯萨基B组病毒 内皮细胞 细胞间粘附分子-1 单克隆抗体
Coxsackie B3 virus infection enhances mononuclear cells adhesion to cultured cardiac microvascular endothelial cells
SHEN Qian, XU Yu-Lian, LING Wei et al.
Department of Clinical Laboratories, Changhai Hospital, Shanghai 200433
Abstract Objective: To investigate the inductive effects of coxsackie B3 virus (Cox B3) infection on ICAM-1 expression of cultured cardiac microvascular endothelial cells and adhesion to mononuclear cells. Methods: Flow cytometry on a FACScan to analyze expression on cardiac microvascular endothelial cells, adhesion assay and monoclonal antibodies inhibition assay to observe the adhesion of mononuclear cells to endothelial cells. Results: Adhesion of rat mononuclear cells to endothelial cells was significantly enhanced following Cox B3 infection for 24 or 48 h (P＜0.01). Cox B3 infection significantly increased ICAM-1 expression on cultured endothelial cells by flow cytometry. Adhesion was reduced by blocking monoclonal antibodies (McAb) to endothlial cells ICAM-1 or mononuclear cells LFA-1, the blocking effect of McAb against ICAM-1 was more obvious than that of McAb to LFA-1. Conclusion: Cox B3 infections of cardiac microvascular endothelia cells can increase surface expression of ICAM-1, and promote inflammatory cell adherence, which could potentially contribute to the cardiac injury of viral myocarditis.
Key words Coxsackie virus B Endothelial cell ICAM-1 Monoclonal antibody
[1] [2] 下一页
上一个医学论文： 竞争性化学发光酶免疫分析法检测睾酮
下一个医学论文： 细胞因子对小鼠 2 I 型前胶原基因启动子活性影响的研究
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文