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去甲斑蝥素诱导人肝癌BEL |
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孙震晓 赵天德 魏育林 李家实
【摘要】 目的 研究新型抗癌药物去甲斑蝥素抑制人肝癌(BEL-7402)细胞生长的机制。 方法 从细胞的形态学观察和DNA琼脂糖凝胶电泳等方面考察去甲斑蝥素作用人肝癌细胞的特点，并进一步用免疫细胞化学及Western blotting等分析手段研究癌基因蛋白Bcl-2在此过程中的表达情况。 结果 10mg/L去甲斑蝥素作用人肝癌细胞24h，部分细胞出现固缩、细胞质膜突起、核染色质异常凝集等，并伴有膜包被的凋亡小体的形成。DNA琼脂糖凝胶电泳显示，处理组部分细胞的DNA已降解成以约200bp为基数的DNA片断，呈现典型的“梯状”带纹。免疫细胞化学及Western blotting分析显示该过程伴随癌基因蛋白Bcl-2表达的减弱。 结论 新型抗癌药物去甲斑蝥素可以诱导人肝癌细胞发生凋亡，该凋亡过程与癌基因蛋白Bcl-2表达下调相关。
【关键词】 去甲斑蝥素 人肝癌细胞 凋亡 Bcl-2
STUDY ON APOPTOSIS OF HUMAN HEPATOMA CELLS (BEL-7402)
INDUCED BY NORCANTHARIDIN
Sun Zhenxiao，Zhao Tiande*，Wei Yulin*，Li Jiashi△
(Liaocheng Teacher s University,Liaocheng 252059;China-Japan Friendship Institute of
Clinical Medical Sciences,Beijing;Beijing University of Traditional Chinese Medicine,Beijing)
【Abstract】 Objective We tried to probe the antitumor mechanisms of new antitumor drug norcantharidin(NCTD) on human hepatoma (BEL-7402) cells. Methods By morphological observation and DNA agarose gel electrophoresis, we examined the characteristics of NCTD on human hepatoma cells, then determined the expression of oncoprotein Bcl-2 by immunocytochemical and Western blotting analysis in this process. Results Treated with 10mg/L NCTD for 24h, some human hepatoma cells condensed with membrane budding, the nuclear chromatin compaction and membrane-enclosed apoptotic bodies formation, and fragments that were multiples of units comprising about 200 base pairs were detected by agarose gel electrophoresis, a characteristic ladder being evident when ethidium bromide-stained gels were viewed in ultraviolet light. The expression of oncoprotein Bcl-2 was proved decreasing in human hepatoma cells at this time. Conclusion The new antitumor drug NCTD can induce apoptosis in human hepatoma cells, and the declining expression of oncoprotein Bcl-2 might involve in this process.
【Key words】 Norcantharidin; Human hepatoma cell; Apoptosis; Bcl-2
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