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表皮生长因子及其受体在食管癌中的表达 |
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丁明杰 丁蔚 银德仁 白经修 张娓 高晓东 张林庆 牛富文 付士显
【摘要】 目的 研究表皮生长因子(epidermal growth factor, EGF)及其受体(EGF receptor, EGFR)在食管鳞状细胞癌中的表达。 方法用免疫组织化学ABC法分析并研究染色结果与病理改变之间的关系。EGF染色，以正常人颌下腺作为阳性对照。EGFR以正常人胎盘组织为阳性对照。EGF和EGFR染色一抗的浓度分别为1∶200，1∶100，4℃孵育48h。 结果 EGF强阳性18例，弱阳性16例，EGFR强阳性18例，弱阳性16例。EGF和EGFR的表达分为两种类型：M型：癌组织本身免疫组织化学反应强弱不同，D型：癌组织中免疫组织化学反应强度均一。D型与M型在EGF、EGFR的表达中较一致。M型较多发生淋巴结转移(P<0.05)，并且强阳性组中M型所占比例较大。 结论 EGF及EGFR强阳性表达同食管癌病人的预后有关。
【关键词】 表皮生长因子(EGF) 表皮生长因子受体(EGFR) 食管癌 免疫组织化学
EXPRESSION OF EPIDERMAL GROWTH FACTOR AND
ITS RECEPTOR IN ESOPHAGEAL CANCER
Ding Mingjie，Ding Wei，Yin Deren*，Bai Jingxiu，Zhang Wei，Gao Xiaodong，
Zhang Linqing，Niu Fuwen，Fu Shixian
(Department of Histology and Embryology, Henan Medical University, Zhengzhou,*Department of Pathology, Xinxiang Central Hospital)
【Abstract】 Objective In order to detect the expression of both EGF and EGFR in human esophageal squamous cell carcinoma. Method The specimens from 34 patients with esophageal cancer were studied by immunohistochemical assay. Results The EGF and EGFR were faintly expressed in basal and parabasal layers of normal esophageal epithelia. No correlation was found between the intensity of EGF、EGFR staining and the histologic type, the patients with strongly expressed EGF and EGFR had lymph node metastases more frequently. The EGF and EGFR expression showed a mosaic (M) pattern in 15 patients and diffuse (D) pattern in 19 patients. The patients with a M pattern had lymph node metastases more frequently than those with a D pattern. In cancer cells, EGFR was stained both in the cytoplasm and on the cell membrane, some of them in the nucleus. Conclusion The overexpression of EGF and EGFR could be of prognostic significance in esophageal cancer.
【Key words】 Epidermal growth factor(EGF); Epidermal growth factor receptor (EGFR); Esophageal carcinoma; Immunohistochemistry
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