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镉对大鼠输精管损伤及锌保护的超微结构与葡萄糖 |
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罗子国 李维信
【摘要】 目的 研究小剂量氯化镉对大鼠输精管近、远段的影响及锌的保护作用。 方法 用1%氯化镉(2mg/kg 体重)腹腔注射雄性Wistar大鼠后4h到60d及镉、锌联合注射后3d、15d取材。采用电镜观察和葡萄糖-6-磷酸酶(G-6-Pase)电镜细胞化学定量研究。 结果 镉注射4h后，输精管主细胞出现超微结构改变，3～7d改变最明显，15d后有所减轻，60d后基本恢复正常。主细胞的主要改变为线粒体肿胀，内质网扩张及髓样结构增多。镉注射后4h，远段主细胞G-6-Pase反应产物明显减少，3d最少，15d反应产物增多。镉、锌联合注射后超微结构及G-6-Pase活性损伤均较单纯镉组轻。 结论 锌对镉所致大鼠输精管主细胞超微结构损伤及远段主细胞G-6-Pase活性影响有明显保护作用。
【关键词】 镉 锌 输精管 超微结构 葡萄糖-6-磷酸酶 大鼠
THE INJURIOUS EFFECTS OF CADMIUM AND PROTECTION
OF ZINC ON ULTRASTRUCTURE AND CYTOCHEMISTRY
OF G-6-Pase OF VAS DEFERENS IN RATS
Luo Ziguo△， Li Weixin
(Laboratory of Electron Microscopy, Chongqing University of Medical Sciences, Chongqing)
【Abstract】 Objective To study the injurious effects of small dose CdCl2 and protection of zinc on proximal segment and distal segment of vas deferens in rats. Methods The rats were treated with CdCl2(2mg/kg body weight) and Cd+Zn. The specimens were investigated by transmission electron microscopy and quantitative analysis of the changes of the cytochemical reactive product of G-6-Pase from 4 hours to 60 days after treatment. Results The ultrastructural changes of principal cells were detected after 4 hours of Cd treatment. The degeneration became most prominent within 3-7 days, but tended towards recovery after 15 days and nearly normal appearance on 60 days after Cd treatment. The main alterations in principal cells were dilatation of endoplasmic reticulum (ER), swelling of mitochondria and increasing of myelin figures. G-6-Pase reactive product of principal cells of distal segment decreased 4 hours after Cd treatment, became most prominent after 3 days, tended to increase after 15 days. The protective effects of zinc on Cd induced injuries in ultrastructure of principal cells and G-6-Pase reactive product of distal segment principal cells from vas deferens were prominent in rats. Conclusion Cadmium had injurious effects on the ultrastructure a[1] [2] 下一页
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