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一个与非小细胞肺癌转移相关的基因 RAB5A基因 |
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李钰 宋岩 陆纲 邹荣 邹亚男 张贵寅 李璞
摘 要： 采用mRNA差异展示技术（mRNA DD）研究具有相同细胞来源，但转移能力高低不同的人肺腺癌细胞系AGZY83-a（低转移）和Anip973(高转移)，分析在两个细胞系中基因差异表达的情况，发现在高转移细胞系中有RAB5A基因的表达。该基因为蛋白质入胞信号的调控者，为RAS超家族成员。为进一步证实其转录表达的调控改变情况，以及RAB5A高表达的临床意义，进一步采用RT-PCR和免疫组织化学的方法检测了50例临床非小细胞肺癌的手术标本，结果表明，RAB5A的表达有随转移发生而增强的趋势，而RAB5A的蛋白表达程度在有转移的病例中明显增强(P<0.05)。
关键词: 肺癌;肿瘤转移;mRNA差异显示;RAB5A基因表达
中图分类号: Q39;R734 文献标识码: A 文章编号: 0253-9772(1999)04-0006-10
A Metastasis-Related Gene with
Non-small Lung Carcinoma——RAB5AGene
Abstract： Using mRNA differential display (mRNA DD)techniques, we analyzed the differences of gene expression between two human lung adenocarcinoma cell lines,AGZY83-a and Anip973. Anip973 was isolated from AGZY83-a, but manifested much higher metastatic potential than the parent line. The results showed that there were significant differences on gene expression between the two cell lines and that there was over-expression of RAB5Agene in the Anip973 cell line. The product of RAB5Agene was recognized as signal regulators of endocytotic pathway and protein trafficking at the cell surface, and belong to a member of the RAS superfamily. Furthemore, we compared to the expression of RAB5Agene and RAB5Aprotein in clinical samples of 50 cases non-small lung carcinoma and nearby lymph node by means of RT-PCR and immunohistochemistry method. The results indicated that the high expression of RAB5Ain metastatic tumor and the enhancement level of RAB5Ain metastatic tumor and the enhancement level of expression were corresponded with the increase of metastatic degree. And there were significance of difference on the expression degree of RAB5Aprotein between non-small lung carcinoma with metastasis and non- metastasis (P<0.05).
Key words： Lung cancer;Tumor metastasis;mRNA differential display;Gene expression of RAB5A
肺癌转移是恶性肿瘤最基本的生物学特征，具有复杂的多阶段过程。在这个复杂的过程中必然有许多基因在不同层次上参与作用。研究与癌细胞转移相关的细胞表型形成的分子基础和癌细胞转移相关的基因调控，分离转移相关[1] [2] 下一页
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