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基底前脑NOS神经元移植至成年鼠海马内的发育 |
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燕启江 姚志彬 周丽华 陈以慈
【摘要】 目的 研究基底前脑NOS神经元移植至成年鼠海马内后，NOS的发育变化规律，同时观察移植物与宿主海马间发生联系的情况。 方法 将大鼠14～16d胚胎的基底前脑移植到单侧穹隆海马伞切断的成年大鼠海马内，动物在移植后存活5、7、14、30、60、90、150和180d分别取脑，经NADPH-d法和尼氏染色观察。 结果 在移植后第7d时NADPH-d阳性染色才出现在NOS阳性神经元内，随着移植物成活时间的延长，NOS阳性神经元逐渐生长在30d时已发育成熟，而且30d时可见其与宿主海马发生联系，直到180d仍有良好的联系。 结论 移植物的NOS阳性神经元在海马的发育有一定的规律。移植物可以在海马内长期存活并可以和宿主脑整合生长，表明移植物可能有功能。
【关键词】 一氧化氮合酶 移植 海马 基底前脑 大鼠
DEVELOPMENT OF NOS NEURONS OF BASAL FOREBRAIN
TRANSPLANTED INTO THE HIPPOCAMPUS
OF ADULT RATS
Yan Qijiang, Yao Zhibin△， Zhou Lihua, Chen Yici
(Department of Anatomy, Division of Brain Research, Sun Yat-sen University of Medical Sciences, Guangzhou)
【Abstract】 Objective To explore the developmental regularity of basal forebrain NOS neurons after transplanted into host hippocampus of adult rats, and observe the graft integrated with host hippocampus. Method The basal forebrain cell suspensions were prepared from tissue obtained from 14－16 day old rat embryos. The cell suspensions were injected into the hippocampus of rat which had received unilateral transection of fimbria-fornix before one week. 5，7，14，30，60，90，150 and 180 days after grafting, the rats were perfused and postfixed, cut section. A series of every second and third sections were used for NOS enzyme histochemistry and Nissl histochemistry. Results The results indicated that grafts could well survive in the hippocampal. NOS was detected in grafted neurons at 7 days after transplantation. Along with survival time, NOS-positive neurons matured at 30 days.30 until 180 days after implantation, NOS positive fibers had expended from the neurons in the grafts into the lesioned hippocampus and dentate gyrus of the host. Conclusion Grafted NOS positive neurons have some growth regularity in host hippocampus. The grafts can long time survive and become integrated with the host brain, indicating that they may be functional in hippoca[1] [2] 下一页
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