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以HRP及氚水作示踪剂研究脑脊液的回流途径 |
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董剑平 孙善全
【摘要】 目的 研究脑脊液(CSF)的正常回流途径。 方法 将HRP、氚水作为示踪剂分别缓慢注入两组大白鼠侧脑室。应用光、电镜技术和液闪、放射自显影技术对HRP和氚水在脑组织中分布状况进行观察。 结果 在脑室周围器官及脑室周围室管膜、软脑膜层有HRP反应产物的聚集，在鼻中隔的鼻粘膜下组织间隙、嗅丝周围间隙及静脉周围和管腔内也可见到HRP反应产物；液闪计数显示，大白鼠的脉络丛、脑垂体、最后区、松果体等脑室周围器官放射性活度明显高于本底；放射自显影显示大白鼠脑组织神经层细胞内有散在分布的银颗粒。 结论 CSF可经由脑室周围器官、脑膜及鼻粘膜下静脉网汇入体循环，同时CSF可回流至大脑神经细胞层，参与形成神经细胞层组织液，与维持脑的正常功能有关。
【关键词】 脑脊液循环 脑室周围器官 鼻粘膜下组织间隙 嗅丝周围间隙
A STUDY ON THE ROUTE OF CSF ABSORPTION BY
USING HRP AND 3H2O AS TRACERS
Dong Jianping, Sun ShanquanΔ
(Chongqing University of Medical Sciences, Chongqing)
【Abstract】 Objective To define the route for the cerebrospinal fluid(CSF) absorption. Methods Horseradish peroxidase(HRP) and 3H2O were injected into the lateral ventricle of two groups of the rats, respectively. To observe the distribution of HRP and 3H2O in the rat brain, light microscopy, electron microscopy, sintillation count and radioautography were applied. Results The choroid plexus, hypophysis, area postrema and pineal body, namely, circumventricular organs(CVOs) and ependyma as well as pia mater layers were stained by the HRP reaction products.The HRP reaction products were observed in the tissue space of membrane mucosa nasi, around the fila olfactoria and venules, and within the venules under the light microscopy and electron microscopy, also. Scintillation count showed that the radioactivity of the CVOs was higher than that of the background. The radioautography showed that the scattered grains of silver existed within the cells of neurocyte layer also. Conclusion The results indicate that the CSF drains into the general circulation through the venules of the CVOs and the meninges of the brain, and the venous plexus in the submucosa of the nasal; and CSF might be play an important role for the metabolism of the brain.
【Key words】 Cerebrospinal fluid circulate; Circumventricular organs; Tissue space of membrane mu[1] [2] 下一页
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