|
丙肝病毒 HCV 基因免疫实验研究 |
| |
邓 涛 陈乃玲 陈天宝 武珊珊 王孟薇 贾克明
中国图书分类号 R322.47 R512.63 R392.1
摘 要 目的：探讨HCV核心区基因疫苗在Balb/c小鼠的免疫应答。方法：将与HCV核心区基因互补的cDNA定向克隆于真核表达载体pcDNA3(pcDNA-HCV)，免疫Balb/c小鼠，观测小鼠血清抗-HCV抗体及小鼠脾细胞对HCV核心抗原的特异性增殖反应。结果：5只经皮下注射的免疫鼠中有4只出现抗-HCV抗体，经肌肉注射的免疫鼠全部出现抗体阳转，而对照组全阴性，肌肉注射免疫组抗体水平高于皮下注射免疫组，动态观察发现追加免疫2 w后抗体即可阳转，4～8 w达高峰，可持续12 w以上。免疫组小鼠脾细胞对HCV核心抗原的刺激指数明显高于对照组。结论：pcDNA-HCV在Balb/c小鼠不仅可诱导体液免疫应答，而且可诱导细胞免疫应答。
关键词 丙肝病毒 基因免疫 核酸疫苗
Plasmid hepatitis C virus core protein:DNA-based immunization for immune responses in Balb/c mice
DENG Tao,CHEN Nai-Ling,CHEN Tian-Bao et al
(Institute of Liver Disease ,Beijing 100700)
Abstract Objective：DNA-based immunization has been proved to be an effective means of vaccination in animal models and may be promising candidates as antiviral agents.There is a need to develope new antiviral agents in an attempt to eradicate persistent HCV infection from liver.In this study,the immune responses to HCV core gene DNA-bassed immunization in Balb/c mice were evaluated.Methods：The HCV core gene cDNA(genotype:II)was inserted into the eukaryotic expression vector under the CMV promoter between EcoRI and Xbal I site (pcDNA-HCV).The Balb/c mice were immunized by multiple sites intramuscular or subcutantous injection with pcDNA-HCV.The anti-HCV Ab in serum were detected by ELISA,meanwhile, the proliferative reponses of spleencytes to HCV core antigens were measured by MTT method.Results：The anti-HCV Ab were detected in 4 out of 5 immunized by subcutaneous injection and in all mice immunized by intramuscular injection.Peek titer were reached by 4～6 weeks after immunization and were maintained for at lease 3 months.The stimulation index of spleencytes from mice immunized with pcDNA-HCV to HCV core antigen were significantly higher than that of mice injected with pcDNA3(P＜0.01).Conclusion：The study demonstrated that both humoral and cellular immune responses could be elicited in mice by pcDNA-HCV.Gene [1] [2] 下一页
上一个医学论文： 小鼠腹水IgM型单抗的纯化
下一个医学论文： 多抗甲素与IL
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 基础医学论文 >> 正文